Rubio S, Chailley-Heu B, Ducroc R, Bourbon J R
INSERM U 319, Université Denis Diderot-Paris, France.
Biochem Biophys Res Commun. 1996 Aug 23;225(3):901-6. doi: 10.1006/bbrc.1996.1270.
An antibody raised against rat pulmonary surfactant protein A (SP-A) bound on Western blots to proteins present in intestinal mucosa and in swim bladder, but not in gills of the carp. The fish protein(s) revealed by the antibody exhibited an electrophoretical behavior similar to that of rat SP-A with a characteristic triplet in the 30- to 35-kDa range. It therefore appears that proteins immunologically very close to mammalian SP-A are present in modern fish which evolved from ancestors that never possessed lungs. The association of SP-A with phospholipid-rich surfactant-like materials appears as a phylogenetically old feature.
一种针对大鼠肺表面活性物质蛋白A（SP-A）产生的抗体，在蛋白质印迹法中与存在于鲤鱼肠黏膜和鱼鳔中的蛋白质结合，但不与鲤鱼鳃中的蛋白质结合。该抗体所揭示的鱼类蛋白质表现出与大鼠SP-A相似的电泳行为，在30至35 kDa范围内有特征性的三联体。因此，在从没有肺的祖先进化而来的现代鱼类中似乎存在与哺乳动物SP-A免疫上非常接近的蛋白质。SP-A与富含磷脂的表面活性剂样物质的关联似乎是一个系统发育上古老的特征。
