Ogura K, Nara K, Watanabe Y, Kohno K, Tai T, Sanai Y
Department of Tumor Immunology, Tokyo Metropolitan Institute of Medical Science, Japan.
Biochem Biophys Res Commun. 1996 Aug 23;225(3):932-8. doi: 10.1006/bbrc.1996.1274.
O-acetylated sialic acids of glycoproteins and gangliosides show cell type-specific and developmentally regulated expression in various systems and sometimes reappear as oncofetal antigens. O-acetylation of sialic acids may influence cell-cell interactions mediated by the sialic acid-binding lectins. Here we describe the molecular cloning and sequencing of a gene that produces O-acetyl disialoganglioside (O-acetyl GD3). Expression analysis showed that this gene product participates the O-acetylation of GD3 ganglioside sialic acid. The deduced amino acid sequence of the product was found to be similar to the milk fat globule membrane glycoprotein.
糖蛋白和神经节苷脂的O-乙酰化唾液酸在各种系统中表现出细胞类型特异性和发育调控表达，有时会作为癌胚抗原重新出现。唾液酸的O-乙酰化可能影响由唾液酸结合凝集素介导的细胞间相互作用。在此，我们描述了一个产生O-乙酰二唾液酸神经节苷脂（O-乙酰GD3）的基因的分子克隆和测序。表达分析表明，该基因产物参与了GD3神经节苷脂唾液酸的O-乙酰化。发现该产物推导的氨基酸序列与乳脂肪球膜糖蛋白相似。
