Dietzmann K, von Bossanyi P, Sallaba J, Kirches E, Synowitz H J, Warich-Kirches M
Department of Neuropathology, Medical School, Otto-von-Geuricke University of Magdeburg, Germany.
Gen Diagn Pathol. 1996 May;141(5-6):339-44.
The aim of this report was to investigate the expression of the p53 and mdm-2 oncoproteins in astrocytic gliomas and to assess their interrelation to proliferating activities. Using monoclonal antibodies directed against p53 and mdm-2, these proteins were stained immunohistochemically in 60 astrocytic brain tumors with different histologic grade. Positive p53 stained nuclei were detected in 25.4% of the tumor cases. Mdm-2 staining products were only localized in 10.5% of specimens. Significant correlations could be found between p53, MIB-1, PCNA and mitotic index on the one hand, and tumor grade on the other hand. There were no clear relations between mdm-2 expression and proliferation markers. The grade of ploidy has a lower priority for the proliferating activity. In most cases mdm-2 immunoreactivity was strongly associated with a low or negative p53 expression.
本报告旨在研究p53和mdm-2癌蛋白在星形胶质细胞瘤中的表达，并评估它们与增殖活性的相互关系。使用针对p53和mdm-2的单克隆抗体，对60例不同组织学分级的星形胶质细胞瘤进行免疫组织化学染色。在25.4%的肿瘤病例中检测到p53阳性染色细胞核。mdm-2染色产物仅在10.5%的标本中定位。一方面，p53、MIB-1、PCNA和有丝分裂指数与另一方面的肿瘤分级之间存在显著相关性。mdm-2表达与增殖标志物之间没有明确关系。倍体等级对增殖活性的影响较小。在大多数情况下，mdm-2免疫反应性与低p53表达或p53阴性表达密切相关。
