Asayama K, Yokota S, Dobashi K, Kawada Y, Nakane T, Kawaoi A, Nakazawa S
Department of Pediatrics, Yamanashi Medical College, Japan.
Histochem Cell Biol. 1996 May;105(5):383-9. doi: 10.1007/BF01463659.
To determine the distribution of cellular glutathione peroxidase in rat lungs, the tissues were stained immunohistochemically. Quantitative analysis was performed in certain cell types of alveolar linings, after the ultrathin sections were stained by a postembedding immunogold technique. Immunoblot analysis revealed that homogenates of rat liver, heart, and lungs all gave a single band. Under the light microscope, the following tissues were stained intensely: epithelial cells, smooth muscle cells and glands of bronchi and bronchioles, type II alveolar cells, and alveolar macrophages. Under immunoelectron microscopy, type II alveolar cells and macrophages were abundant in mitochondria. The mitochondria, nucleus, and cytoplasm of macrophages were labeled almost twice as densely as the respective compartments of type II alveolar cells. Within cell types, the mitochondria were labeled twice as densely as the nuclei. The other particles were less than half as densely labeled as the nuclei. The labeling was slightly less dense in the cytoplasm than in the nucleus. The present study revealed that glutathione peroxidase occurred predominantly in the epithelial linings and metabolically active sites in rat lungs. The tissues that were previously found to be rich in superoxide dismutases were also rich in glutathione peroxidase.
为了确定细胞谷胱甘肽过氧化物酶在大鼠肺中的分布，对组织进行了免疫组织化学染色。在超薄切片通过包埋后免疫金技术染色后，对肺泡内衬的某些细胞类型进行了定量分析。免疫印迹分析显示，大鼠肝脏、心脏和肺的匀浆均呈现单一条带。在光学显微镜下，以下组织染色强烈：支气管和细支气管的上皮细胞、平滑肌细胞和腺体、II型肺泡细胞和肺泡巨噬细胞。在免疫电子显微镜下，II型肺泡细胞和巨噬细胞的线粒体丰富。巨噬细胞的线粒体、细胞核和细胞质的标记密度几乎是II型肺泡细胞相应区域的两倍。在细胞类型中，线粒体的标记密度是细胞核的两倍。其他颗粒的标记密度不到细胞核的一半。细胞质中的标记密度略低于细胞核。本研究表明，谷胱甘肽过氧化物酶主要存在于大鼠肺的上皮内衬和代谢活跃部位。先前发现富含超氧化物歧化酶的组织也富含谷胱甘肽过氧化物酶。
