Cook J E, Kondrashev S L, Podugolnikova T A
Department of Anatomy and Developmental Biology, University College London, United Kingdom.
Vis Neurosci. 1996 May-Jun;13(3):517-28. doi: 10.1017/s0952523800008191.
Biplexiform ganglion cells were labelled by retrograde transport of HRP in five species of marine fish from the neoteleost acanthopterygian orders Perciformes and Scorpaeniformes. Their forms and spatial distributions were studied in retinal flatmounts and thick sections. Biplexiform ganglion cells possessed sparsely branched, often varicose, dendrites that ramified through the inner nuclear layer (INL) to reach the outer plexiform layer (OPL), as well as conventional arborizations in the most sclerad part of the inner plexiform layer (IPL). Their somata were of above-average size and were displaced into the vitread border of the INL. Mean soma areas ranged from 99 +/- 6 microns2 in Bathymaster derjugini (Perciformes) to 241 +/- 12 microns2 in Hexagrammos stelleri (Scorpaeniformes), but were similar in each species to those of the outer-stratified alpha-like ganglion cells, whose dendritic trees occupied the same IPL sublamina. In the best-labelled specimens, biplexiform cells formed clear mosaics with spacings and degrees of regularity much like those of other large ganglion cells, but spatially independent of them. Biplexiform mosaics were plotted in three species, and analyzed by nearest-neighbor distance and spatial correlogram methods. The exclusion radius, an estimate of minimum mosaic spacing, ranged from 113 microns in Hexagrammos stelleri, through 150 microns in Ernogrammus hexagrammus (Perciformes), to 240 microns in Myoxocephalus stelleri (Scorpaeniformes). A spatial cross-correlogram analysis of the distributions of biplexiform and outer-stratified alpha-like cells in Hexagrammos demonstrated the spatial independence of their mosaics. Similar cells were previously observed not only in the freshwater cichlid Oreochromis spilurus (Perciformes) but also in the goldfish Carassius auratus (Cypriniformes) which, being an ostariophysan teleost, is only distantly related. Thus, biplexiform ganglion cells may be regular elements of all teleost fish retinae. Their functional role remains unknown.
通过辣根过氧化物酶（HRP）逆行运输，在新真骨鱼棘鳍目鲈形目和鲉形目的5种海鱼中标记了双极神经节细胞。在视网膜平铺标本和厚切片中研究了它们的形态和空间分布。双极神经节细胞具有稀疏分支、常呈曲张状的树突，这些树突穿过内核层（INL）到达外网状层（OPL），并且在内网状层（IPL）最硬脑膜侧部分有传统的分支。它们的胞体大小高于平均水平，并向内核层的玻璃体边界移位。胞体平均面积范围从德氏深海鲈（鲈形目）的99±6平方微米到斯氏六线鱼（鲉形目）的241±12平方微米，但在每个物种中与外层分层的α样神经节细胞相似，其树突占据相同的内网状层亚层。在标记最好的标本中，双极细胞形成了清晰的镶嵌图案，其间距和规则程度与其他大型神经节细胞非常相似，但在空间上与它们独立。在三个物种中绘制了双极镶嵌图案，并通过最近邻距离和空间相关图方法进行分析。排除半径（最小镶嵌间距的估计值）范围从斯氏六线鱼的113微米，到六线纹鲈（鲈形目）的150微米，再到斯氏杜父鱼（鲉形目）的240微米。对六线鱼中双极细胞和外层分层α样细胞分布的空间交叉相关图分析表明它们镶嵌图案的空间独立性。之前不仅在淡水丽鱼奥氏非鲫（鲈形目）中观察到类似细胞，而且在金鱼鲫（鲤形目）中也观察到，金鱼作为骨鳔总目硬骨鱼，与上述物种亲缘关系较远。因此，双极神经节细胞可能是所有硬骨鱼视网膜的常规组成部分。它们的功能作用仍然未知。
