Connan R M
Vet Rec. 1977 May 14;100(20):421-2. doi: 10.1136/vr.100.20.421.
Eggs were collected from gravid female Ascaris suum in the abattoir. Cultures were prepared with the eggs in 2 to 3 mm depth of 1 per cent sulphuric acid or on the surface of 2% aqueous agar. They were allowed to develop in a piggery, fresh cultures being started at intervals from January to September. Eggs on the surface of agar developed failry uniformly and a high proportion eventually reached L2. In contrast those under 1% sulphuric acid were less uniform, slower in development and generally less successful. Cultures started on agar between January and May, or from September became infective at approximately the same time in July while those started during June and July contained infective eggs in August and September respectively. No recognisable development occurred between October and April. The significance of these findings for the epidemiology of ascariasis is discussed.
在屠宰场从怀孕的雌性猪蛔虫中收集虫卵。将虫卵置于2至3毫米深的1%硫酸中或2%水琼脂表面制备培养物。它们在养猪场中发育,从1月至9月定期开始新的培养。琼脂表面的虫卵发育较为均匀,最终很大比例的虫卵发育到L2期。相比之下,1%硫酸下的虫卵发育不太均匀,发育较慢且总体成功率较低。1月至5月或9月在琼脂上开始的培养物在7月大致同时具有感染性,而6月和7月开始的培养物分别在8月和9月含有感染性虫卵。10月至4月未观察到明显的发育。讨论了这些发现对蛔虫病流行病学的意义。