Kordick D L, Swaminathan B, Greene C E, Wilson K H, Whitney A M, O'Connor S, Hollis D G, Matar G M, Steigerwalt A G, Malcolm G B, Hayes P S, Hadfield T L, Breitschwerdt E B, Brenner D J
Department of Companion Animal and Special Species Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
Int J Syst Bacteriol. 1996 Jul;46(3):704-9. doi: 10.1099/00207713-46-3-704.
Two bacterial strains, one isolated from the blood of a dog with valvular endocarditis and one isolated from the blood of a healthy dog, were similar to Bartonella species, as determined by a number of phenotypic criteria, including growth characteristics, biochemical reactions, and cell wall fatty acid composition. The results of 16S rRNA gene sequence similarity studies confirmed that these strains are closely related and belong in the genus Bartonella and that Bartonella vinsonii is their closest relative (the 16S rRNA of isolate 93-C01T [T = type strain] was 99.37% identical to the 16S rRNA of the type strain of B. vinsonii, the 16S rRNA of isolate G7464 was 99.61% identical to the 16S rRNA of the type strain, and the 16S rRNAs of the dog isolates were 99.77% identical to each other). The 16S rRNAs of both strains contained a 12-base insertion that was not present in the 16S rRNA of the type strain of any Bartonella species. DNA relatedness tests revealed that these strains were related at the species level to the type strain of B. vinsonii. They were, however, significantly more closely related to each other than to B. vinsonii. On the basis of their unique 16S rRNA sequence insertion, their preferentially high level of relatedness, and their similar origins (dogs), we believe that strains 93-C01(T) and G7464 should be placed in a separate subspecies of B. vinsonii, for which we propose the name B. vinsonii subsp. berkhoffii subsp. nov. The type strain of B. vinsonii subsp. berkhoffii is strain 93-C01 (= ATCC 51672). The description of B. vinsonii is emended to accommodate the new subspecies, and B. vinsonii subsp. vinsonii is described.
通过一系列表型标准，包括生长特性、生化反应和细胞壁脂肪酸组成测定，从患有瓣膜性心内膜炎的犬血液中分离出的一株细菌和从健康犬血液中分离出的另一株细菌与巴尔通体属菌种相似。16S rRNA基因序列相似性研究结果证实，这些菌株密切相关，属于巴尔通体属，且文森巴尔通体是它们最接近的亲缘种（分离株93-C01T [T = 模式菌株] 的16S rRNA与文森巴尔通体模式菌株的16S rRNA有99.37% 的同源性，分离株G7464的16S rRNA与模式菌株的16S rRNA有99.61% 的同源性，犬分离株的16S rRNA彼此间有99.77% 的同源性）。两株菌的16S rRNA均含有一个12碱基的插入序列，该序列不存在于任何巴尔通体菌种模式菌株的16S rRNA中。DNA相关性测试表明，这些菌株在种水平上与文森巴尔通体模式菌株相关。然而，它们彼此之间的相关性明显高于与文森巴尔通体的相关性。基于它们独特的16S rRNA序列插入、优先的高度相关性以及相似的来源（犬），我们认为菌株93-C01(T) 和G7464应归入文森巴尔通体的一个单独亚种，我们为此提议命名为文森巴尔通体伯克霍夫亚种（Bartonella vinsonii subsp. berkhoffii）。文森巴尔通体伯克霍夫亚种的模式菌株是93-C01菌株（= ATCC 51672）。对文森巴尔通体的描述进行了修订以纳入新亚种，并描述了文森巴尔通体文森亚种（Bartonella vinsonii subsp. vinsonii）。
