Colder B W, Wilson C L, Frysinger R C, Harper R M, Engel J
Center for Molecular and Behavioural Neuroscience, Rutgers University, Newark, NJ 07102, USA.
Brain Res. 1996 May 6;719(1-2):96-103. doi: 10.1016/0006-8993(96)00107-2.
We recorded 259 single neurons from mesial temporal lobe structures of 21 patients with complex partial seizures. Interspike intervals within clusters of action potentials (clustered interspike intervals) recorded from cells in mesial temporal structures ipsilateral to seizure initiation were compared to clustered interspike intervals in the contralateral temporal lobe. 'Clusters' were defined as any group of three or more spikes separated by intervals of less than a defined maximum, or two spikes separated by less than half that maximum. The maximum interspike interval which defined a cluster was varied from 5 to 40 ms in 5-ms steps. Significantly smaller proportions of clustered spikes were discharged by neurons in the amygdala, hippocampus and entorhinal cortex from the temporal lobe commonly initiating seizures, compared to neurons in contralateral homotopic regions. When data from the same three structures were combined, significantly fewer cluster interspike intervals between 10 and 25 ms were recorded from cells on the side of seizure onset. Because clustered action potential discharge is a normal pattern of firing for cells that discharge endogenous bursts, the relative decrease in proportions of 10-25 ms clustered interspike intervals occurring in the temporal lobe initiating seizures might reflect a reduction in endogenous burst discharges from that side. Reduced endogenous bursting could be due to the loss of burst discharging neurons as a product of seizure-related excitotoxicity. The identification of decreased interictal single neuronal burst discharge in epileptogenic structures stresses the difference between the interictal and ictal states in patients with complex partial seizures, and the importance of the transition between those states.
我们记录了21例复杂部分性癫痫患者内侧颞叶结构中的259个单神经元。将癫痫起始同侧内侧颞叶结构中细胞记录的动作电位簇内的峰间期（簇状峰间期）与对侧颞叶中的簇状峰间期进行比较。“簇”定义为任何一组三个或更多个峰，其间隔小于定义的最大值，或两个峰的间隔小于该最大值的一半。定义簇的最大峰间期以5毫秒的步长从5毫秒变化到40毫秒。与对侧同位区域的神经元相比，通常引发癫痫的颞叶杏仁核、海马体和内嗅皮质中的神经元释放出显著更少比例的簇状峰。当将来自相同三个结构的数据合并时，癫痫发作起始侧的细胞记录到的10至25毫秒之间的簇状峰间期明显更少。由于簇状动作电位发放是内源性猝发放电细胞的正常放电模式，在引发癫痫的颞叶中10 - 25毫秒簇状峰间期比例的相对降低可能反映了该侧内源性猝发放电的减少。内源性猝发减少可能是癫痫相关兴奋性毒性导致猝发放电神经元丧失的结果。在致痫结构中确定发作间期单个神经元猝发放电减少强调了复杂部分性癫痫患者发作间期和发作期状态之间的差异，以及这些状态之间转变的重要性。
