Stargell L A, Struhl K
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Trends Genet. 1996 Aug;12(8):311-5. doi: 10.1016/0168-9525(96)10028-7.
Transcriptional activation involves the regulated assembly of multiprotein complexes on promoter DNA in the context of the repressive effects of chromatin. How do activators orchestrate this complicated phenomenon in vivo? Recent genetic and biochemical advancements suggest that activator-dependent formation of the transcription machinery on the promoter involves at least two steps. First, the activator facilitates the recruitment of TFIID to the TATA element of the promoter. TFIID binding is then followed by the recruitment of the remainder of the transcriptional apparatus in the form of the RNA polymerase II holoenzyme.
转录激活涉及在染色质的抑制作用背景下，多蛋白复合物在启动子DNA上的有序组装。激活因子如何在体内协调这一复杂现象？最近的遗传学和生物化学进展表明，激活因子依赖的转录机器在启动子上的形成至少涉及两个步骤。首先，激活因子促进TFIID募集到启动子的TATA元件上。TFIID结合之后，其余转录装置以RNA聚合酶II全酶的形式被募集。
