Quarta C, Borghi A, Zerilli L F, De Pietro M T, Ferrari P, Trani A, Lancini G C
Hoechst Marion Roussel, Lepetit Research Center, Gerenzano, VA, Italy.
J Antibiot (Tokyo). 1996 Jul;49(7):644-50. doi: 10.7164/antibiotics.49.644.
A new teicoplanin-like antibiotic was discovered when using Actinoplanes teichomyceticus strain 3/W, the fermentation medium containing Alburex, and the fermentation time 275 hours. The new product was separated from teicoplanin complex by polyamide resin chromatography and purified by Amberlite XAD-7 and affinity resin chromatographies. The structure was established on the basis of the physico-chemical characteristics of the complex and of its aglycone. The new structure is that of teicoplanin with a carbonyl group substituting for the CHNH2 group of amino acid 1. We hypothesize that the novel complex is a transformation product of teicoplanin due to a simple transamination reaction, as supported by its structure and by the concomitant decrease in teicoplanin concentration during its production.
当使用游动放线菌3/W菌株、含有Alburex的发酵培养基以及275小时的发酵时间时，发现了一种新的替考拉宁样抗生素。该新产品通过聚酰胺树脂色谱法从替考拉宁复合物中分离出来，并通过Amberlite XAD - 7和亲和树脂色谱法进行纯化。根据该复合物及其糖苷配基的物理化学特性确定了其结构。新结构是替考拉宁的结构，其中一个羰基取代了氨基酸1的CHNH2基团。我们推测这种新型复合物是替考拉宁通过简单的转氨反应产生的转化产物，其结构以及生产过程中替考拉宁浓度的同时降低都支持了这一推测。
