Hyman S E, Borsook D
Laboratory of Molecular and Developmental Neuroscience, Massachusetts General Hospital, Charlestown 02129, USA.
NIDA Res Monogr. 1996;161:59-71.
This chapter gives an overview of the utility of one type of transgenic mouse model for substance abuse research, the use of transgenes in which genomic regulatory sequences of interest are coupled to a reporter gene. Using this mouse model, exploration of the mechanisms regulating expression of the proenkephalin gene in the mouse hypothalamus have begun. The resulting information supplements experiments on gene regulation performed in cell lines with information about regulation by pharmacologic and physiologic stimuli of interest within the brain. With special reference to mechanisms of opioid dependence, the particular model that has been examined appears to be a useful tool to investigate mechanistic aspects of the regulation of endogenous opioid genes by exogenous opioids.
本章概述了一种用于药物滥用研究的转基因小鼠模型的效用，即使用将感兴趣的基因组调控序列与报告基因偶联的转基因。利用这种小鼠模型，已经开始探索调节小鼠下丘脑前脑啡肽基因表达的机制。所得到的信息为在细胞系中进行的基因调控实验提供了补充，这些补充信息涉及大脑中感兴趣的药理和生理刺激的调控。特别是关于阿片类药物依赖的机制，所研究的特定模型似乎是一种有用的工具，可用于研究外源性阿片类药物对内源性阿片类基因调控的机制方面。
