Hamamoto Y, Nakajima T, Ozawa H, Uchida T
First Department of Oral and Maxillofacial Surgery, Niigata University School of Dentistry, Japan.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1996 Jun;81(6):703-9. doi: 10.1016/s1079-2104(96)80077-1.
To study the multiple potentials of differentiating odontogenic epithelial cells.
Bilateral first and second maxillary molars of 30 immature rats were perforated into the pulp chambers with a round bur. The pulps were observed histologically and immunohistochemically for amelogenin 3, 7, and 14 days after the perforation.
On day 7, the enamel epithelium of Hertwig's root sheath migrated and formed oval-shaped epithelial islands that resembled epithelial rests of Malassez. On day 14, the islands consisted of epithelial cells with large nuclei and cytoplasm partially surrounded by a subsequently formed osteodentin and cementum. Immunoreactivity for amelogenin was observed in the large epithelial cells and in the area between the cells as well as the calcified tissues. Some of the enamel epithelium resembling columnar ameloblasts of the enamel organ were also positive for amelogenin.
The enamel epithelium of Hertwig's root sheath appeared to differentiate into ameloblasts and produce amelogenin.
研究牙源性上皮细胞的多种分化潜能。
用圆钻在30只未成熟大鼠的双侧上颌第一和第二磨牙上穿通至髓腔。在穿孔后3、7和14天对牙髓进行组织学和免疫组织化学观察，检测釉原蛋白。
第7天，赫特维希上皮根鞘的釉质上皮迁移并形成椭圆形上皮岛，类似马拉瑟上皮剩余。第14天，这些岛屿由大细胞核和部分被随后形成的骨牙本质和牙骨质包围的细胞质的上皮细胞组成。在大的上皮细胞、细胞间区域以及钙化组织中观察到釉原蛋白的免疫反应性。一些类似釉器柱状成釉细胞的釉质上皮也对釉原蛋白呈阳性反应。
赫特维希上皮根鞘的釉质上皮似乎分化为成釉细胞并产生釉原蛋白。
