羟基磷灰石微晶对巨噬细胞活性的影响。

Effect of hydroxyapatite microcrystals on macrophage activity.

作者信息

Fukuchi N, Akao M, Sato A

机构信息

Division of Inorganic Materials, Tokyo Medical and Dental University, Japan.

出版信息

Biomed Mater Eng. 1995;5(4):219-31.

PMID:8785507

Abstract

Hydroxyapatite (HAp) microcrystals were synthesized by a neutralization reaction of Ca(OH)2 suspension and H3PO4 solution using an ultrasonic homogenizer. The in vitro interaction of HAp microcrystals with rat peritoneal macrophages was investigated by measuring the viability, acid phosphatase (ACP) activity, lactate dehydrogenase (LDH) activity and intracellular calcium content. HAp calcined at 800 degrees C and alpha-alumina particles (alumina) were used as comparative materials. Macrophages actively phagocytosed HAp microcrystals by dissolving them. However, no damage in macrophages exposed to HAp microcrystals was observed by transmission electron microscopy. Macrophages in the presence of HAp microcrystals showed less ACP and LDH activity and higher intracellular calcium content than those in the presence of calcined HAp and alumina. HAp microcrystals had excellent biocompatibility to macrophages as well as sintered HAp.

摘要

采用超声均质器，通过Ca(OH)₂悬浮液与H₃PO₄溶液的中和反应合成了羟基磷灰石（HAp）微晶。通过测量细胞活力、酸性磷酸酶（ACP）活性、乳酸脱氢酶（LDH）活性和细胞内钙含量，研究了HAp微晶与大鼠腹膜巨噬细胞的体外相互作用。以800℃煅烧的HAp和α-氧化铝颗粒（氧化铝）作为对照材料。巨噬细胞通过溶解HAp微晶来积极吞噬它们。然而，透射电子显微镜观察未发现暴露于HAp微晶的巨噬细胞有损伤。与存在煅烧HAp和氧化铝的巨噬细胞相比，存在HAp微晶的巨噬细胞显示出较低的ACP和LDH活性以及较高的细胞内钙含量。HAp微晶与烧结HAp一样，对巨噬细胞具有优异的生物相容性。