促甲状腺激素释放激素对豚鼠离体盲肠环形平滑肌细胞的直接抑制作用。

Direct inhibitory effect of thyrotropin-releasing hormone on isolated cecal circular smooth muscle cells of guinea pig.

作者信息

Harada N, Chijiiwa Y, Akiho H, Okabe H, Nawata H

机构信息

Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

出版信息

Digestion. 1996;57(2):79-82. doi: 10.1159/000201317.

DOI:10.1159/000201317

PMID:8786004

Abstract

Smooth muscle cells isolated from cecal circular smooth muscle of the guinea pig were used to determine whether thyrotropin-releasing hormone (TRH) can inhibit the contractile response produced by 10(-6) M carbachol by exerting a direct action on muscle cells. In addition, the inhibitory effect of 2',5'-dideoxyadenosine (an inhibitor of adenylate cyclase), phorbol 12-myristate 13-acetate (an inhibitor of particulate guanylate cyclase), 6-anilinoquinoline-5,8-quinone (an inhibitor of nitric oxide synthase) on the TRH-induced relaxation of cecal circular smooth muscle cells was examined. TRH inhibited the contractile response produced by 10(-6) M carbachol in a concentration-dependent manner, with an IC50 value of 4 nM, 2',5'-Dideoxyadenosine and phorbol 12-myristate 13-acetate did not have any significant effect on the TRH-induced relaxation. On the other hand, 6-anilinoquinoline-5,8-quinone and N omega-nitro-L-arginine methyl ester significantly inhibited the relaxation produced by TRH. Our findings show that TRH has a direct inhibitory effect on the isolated cecal circular smooth muscle cells via activation of nitric oxide synthase and soluble guanylate cyclase.

摘要

从豚鼠盲肠环行平滑肌中分离出的平滑肌细胞，用于确定促甲状腺激素释放激素（TRH）是否可通过对肌肉细胞发挥直接作用，来抑制由10⁻⁶ M卡巴胆碱产生的收缩反应。此外，还检测了2',5'-二脱氧腺苷（一种腺苷酸环化酶抑制剂）、佛波醇12-肉豆蔻酸酯13-乙酸酯（一种颗粒型鸟苷酸环化酶抑制剂）、6-苯胺基喹啉-5,8-醌（一种一氧化氮合酶抑制剂）对TRH诱导的盲肠环行平滑肌细胞舒张的抑制作用。TRH以浓度依赖的方式抑制10⁻⁶ M卡巴胆碱产生的收缩反应，IC50值为4 nM，2',5'-二脱氧腺苷和佛波醇12-肉豆蔻酸酯13-乙酸酯对TRH诱导的舒张没有任何显著影响。另一方面，6-苯胺基喹啉-5,8-醌和Nω-硝基-L-精氨酸甲酯显著抑制TRH产生的舒张。我们的研究结果表明，TRH通过激活一氧化氮合酶和可溶性鸟苷酸环化酶，对分离出的盲肠环行平滑肌细胞具有直接抑制作用。