Caenaro G F, Caberlotto L, Chirillo R
Istituto di Chimica Clinica, Ospedale Generale Regionale, Treviso.
Minerva Endocrinol. 1996 Mar;21(1):37-42.
The Abbott IMx assay is an affinity method, based on boronate binding to glycated hemoglobin. A novel separation system, ion capture technology, is used for separation of GHb from non-GHb. Affinity-based GHb methods are reported not to be subject to interference from either aldimine intermediates (pre-Alc fraction) or Hb variants that can comigrate in ion-exchange based chromatographic methods with the HbAlc fraction or with HbAO, having, in both cases, an unreliable % HbA1c result. The purpose of the study was to investigate the analytical performance of the GHb IMx assay, illustrating also a case study involving an abnormal hemoglobin variant.
雅培IMx检测法是一种基于硼酸酯与糖化血红蛋白结合的亲和方法。一种新型分离系统——离子捕获技术，用于从非糖化血红蛋白中分离糖化血红蛋白。据报道，基于亲和的糖化血红蛋白检测方法不受醛亚胺中间体（前醛糖血红蛋白部分）或血红蛋白变体的干扰，这些物质在基于离子交换的色谱法中可能与糖化血红蛋白部分或血红蛋白AO一同迁移，在这两种情况下，糖化血红蛋白A1c的百分比结果都不可靠。本研究的目的是调查糖化血红蛋白IMx检测法的分析性能，同时还展示了一个涉及异常血红蛋白变体的案例研究。
