Mochizuki T, Uehara M, Menon T, Ranganathan S
Department of Dermatology, Shiga University of Medical Science, Japan.
Mycoses. 1996 Jan-Feb;39(1-2):31-5. doi: 10.1111/j.1439-0507.1996.tb00080.x.
Total cellular DNA was extracted from 18 isolates of Trichophyton mentagrophytes and six isolates of unidentified Trichophyton species taken from tinea corporis, tinea cruris and tinea pedis patients by a minipreparation method. The DNA was digested with the restriction enzymes HaeIII and MspI, followed by electrophoresis on agarose gels. The restriction enzyme profiles of the DNAs were compared with those of mitochondrial (mt) DNA extracted from reference strains of T. mentagrophytes (Arthroderma simii, A. benhamiae and A. vanbreuseghemii) and T. rubrum. All 18 T. mentagrophytes isolates showed the same DNA profiles as mtDNA from A. vanbreuseghemii. The six unidentified Trichophyton isolates exhibited DNA profiles the same as that of mtDNA from T. rubrum. Consequently, these isolates were identified as T. rubrum. In conclusion, restriction enzyme analysis of total cellular DNA prepared by the minipreparation method is considered to be useful as an alternative method of mtDNA analysis and is helpful for the identification of these dermatophyte species.
采用微量制备法从18株须癣毛癣菌分离株以及从体癣、股癣和足癣患者身上获取的6株未鉴定的毛癣菌分离株中提取总细胞DNA。用限制性内切酶HaeIII和MspI消化DNA，然后在琼脂糖凝胶上进行电泳。将这些DNA的限制性酶切图谱与从须癣毛癣菌（猴状表皮癣菌、贝氏表皮癣菌和范布芮须癣菌）和红色毛癣菌的参考菌株中提取的线粒体（mt）DNA的图谱进行比较。所有18株须癣毛癣菌分离株显示出与范布芮须癣菌的mtDNA相同的DNA图谱。这6株未鉴定的毛癣菌分离株呈现出与红色毛癣菌的mtDNA相同的DNA图谱。因此，这些分离株被鉴定为红色毛癣菌。总之，通过微量制备法制备的总细胞DNA的限制性酶切分析被认为是一种有用的mtDNA分析替代方法，有助于这些皮肤癣菌物种的鉴定。
