1H-15N backbone resonance assignments of bacteriorhodopsin.

Des-(232-248)-bacteriorhodopsin was solubilized in a membrane mimicking environment of methanol-chloroform (1:1) containing 0.1 M 2HCO2N2H4 and 1H-15N backbone resonance assignment was obtained using 2D HMQC, 3D NOESY-HMQC, 3D TOCSY-HMQC and 3D HMQC-NOESY-HMQC NMR experiments. 87 cross-peaks out of 117 present in the HMQC spectrum were assigned to particular residues in 1-73 and 195-231 parts of the protein. For these residues also signals of C alpha H and C beta H protons were assigned.