Grabchuk I A, Arseniev A S
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Pharm Acta Helv. 1996 Jun;71(1):97-102. doi: 10.1016/0031-6865(95)00055-0.
Des-(232-248)-bacteriorhodopsin was solubilized in a membrane mimicking environment of methanol-chloroform (1:1) containing 0.1 M 2HCO2N2H4 and 1H-15N backbone resonance assignment was obtained using 2D HMQC, 3D NOESY-HMQC, 3D TOCSY-HMQC and 3D HMQC-NOESY-HMQC NMR experiments. 87 cross-peaks out of 117 present in the HMQC spectrum were assigned to particular residues in 1-73 and 195-231 parts of the protein. For these residues also signals of C alpha H and C beta H protons were assigned.
去（232 - 248）-细菌视紫红质溶解于含有0.1 M 2HCO₂N₂H₄的甲醇 - 氯仿（1:1）模拟膜环境中，并使用二维HMQC、三维NOESY - HMQC、三维TOCSY - HMQC和三维HMQC - NOESY - HMQC核磁共振实验获得了¹H - ¹⁵N主链共振归属。HMQC谱中117个峰中的87个交叉峰被归属到蛋白质1 - 73和195 - 231部分的特定残基。对于这些残基，还归属了CαH和CβH质子的信号。
