Obata T, Yamanaka Y
Department of Pharmacology, Oita Medical University, Japan.
Biochem Pharmacol. 1996 May 17;51(10):1411-3. doi: 10.1016/0006-2952(96)84524-2.
We examined the effect of iron (II) on the generation of hydroxyl radicals (.OH) in the extracellular fluid of rat myocardium. Salicylic acid in Ringer's solution (0.5 nmole x microliter(-1) x min(-1)) was directly infused through a microdialysis probe to detect the generation of OH as reflected by the formation of dihydroxybenzoic acid (DHBA) in the myocardium. Iron clearly produced a dose-dependent increase in .OH formation. A positive linear correlation between iron (II) and the formation of 2,3-DHBA (R2 = 0.970) or 2.5-DHBA (R2 = 0.983) was observed. However, when desferrioxamine (DES) was infused through a dialysis probe, a marked increase in DHBA formation was obtained. The present results suggest that iron (III) may reduce .OH formation by the Fenton reaction.
