Kondo T, Dollé P, Zákány J, Duboule D
Department of Zoology and Animal Biology, University of Geneva, Switzerland.
Development. 1996 Sep;122(9):2651-9. doi: 10.1242/dev.122.9.2651.
Vertebrate 5'-located HoxD genes are expressed in the most caudal part of the digestive tract and their potential functions during gut development have been assessed by gene disruptions. We have inserted reporter lacZ sequences within the Hoxd-12 gene and analysed the morphology of the gut in these mice as well as in Hoxd-13 mutant animals. When homozygous, both mutations induce an important disorganization of the anorectal region. In particular, severe alterations of the smooth muscle layers of the rectum led to defective morphogenesis of the internal anal sphincter. Similarly, Hoxd-12 and Hoxd-13 functionally overlap during digit development. The function of these genes in the morphogenesis of the digestive system as well as their functional evolution are discussed.
脊椎动物位于5'端的HoxD基因在消化道最尾端部分表达，并且通过基因敲除对它们在肠道发育过程中的潜在功能进行了评估。我们已将报告基因lacZ序列插入Hoxd - 12基因中，并分析了这些小鼠以及Hoxd - 13突变动物的肠道形态。当为纯合子时，两种突变都会导致肛门直肠区域严重紊乱。特别是，直肠平滑肌层的严重改变导致肛门内括约肌形态发生缺陷。同样，Hoxd - 12和Hoxd - 13在指（趾）发育过程中功能重叠。本文讨论了这些基因在消化系统形态发生中的功能及其功能进化。
