Tatsumi H, Tsuji S, Anglade P, Motelica-Heino I, Soeda H, Katayama Y
Department of Autonomic Physiology, Tokyo Medical and Dental University, Japan.
Neurosci Lett. 1995 Dec 29;202(1-2):25-8. doi: 10.1016/0304-3940(95)12187-0.
Neurons from the nucleus diagonal band of Broca (DBB) from new born rats protrude neuronal processes and growth cones in culture. Cytochemical observations with the light and electron microscope indicate that growth cones of these neurons take up choline, synthesize acetylcholine (ACh) and store ACh in the vesicles. Electrical stimuli at the soma of DBB neurons evoked inward currents in ACh-sensitive neurons attached to DBB growth cones. These currents were suppressed by TTX, a Ca2+ channel blocker (Cd2+), and an ACh nicotinic antagonist (C6). These results suggest that ACh is synthesized, stored and released from the growth cones of DBB neurons prior to synapse formation.
新生大鼠布罗卡斜角带核（DBB）的神经元在培养中会伸出神经突起和生长锥。光镜和电镜的细胞化学观察表明，这些神经元的生长锥摄取胆碱，合成乙酰胆碱（ACh）并将ACh储存在囊泡中。对DBB神经元胞体的电刺激在附着于DBB生长锥的ACh敏感神经元中诱发内向电流。这些电流被河豚毒素（TTX）、一种Ca2+通道阻滞剂（Cd2+）和一种ACh烟碱拮抗剂（C6）抑制。这些结果表明，ACh在突触形成之前就从DBB神经元的生长锥中合成、储存和释放。
