Prentø P
Department of Pathology 134, University of Copenhagen, Hvidovre, Denmark.
Histochem J. 1995 Nov;27(11):906-13.
The paper takes issue with the use by glutaraldehyde suppliers of the term 'for electron microscopy', and the common practice of researchers giving insufficient or no data about the glutaraldehyde they use. Investigation of 11 commercial glutaraldehydes recommended for electron microscopy shows that only three or four of them are adequate for this purpose, using criteria set forth in papers dated between 1965 and 1989. The present paper reports that a check of purity can best be done by spectrophotometry. The 234/280 or 235/280 nm absorbance ratio is a precise indicator of the degree of polymerization, provided certain conditions stated in this paper are fulfilled. Some of the storage precautions taken by, or proposed by, suppliers are superfluous, and only mask the inadequate purification by the suppliers. A simple protocol for the storage of stock solutions is given. Alkaline glutaraldeyhyde is inherently very unstable, even in the refrigerator. Fixatives should, therefore, be stored in the freezer or should be freshly prepared.
本文对戊二醛供应商使用“用于电子显微镜检查”这一术语，以及研究人员对其所使用的戊二醛提供的数据不足或未提供数据的常见做法提出了质疑。对11种推荐用于电子显微镜检查的市售戊二醛进行的调查表明，根据1965年至1989年间发表的论文中规定的标准，其中只有三四种适用于此目的。本文报道，纯度检查最好通过分光光度法进行。只要满足本文所述的某些条件，234/280或235/280nm吸光度比是聚合度的精确指标。供应商采取的或建议的一些储存预防措施是多余的，只是掩盖了供应商纯化不足的问题。给出了一个储存储备溶液的简单方案。碱性戊二醛本质上非常不稳定，即使在冰箱中也是如此。因此，固定剂应储存在冰箱中或应新鲜配制。
