Huang Y L, Säljö A, Suneson A, Hansson H A
Institute of Anatomy and Cell Biology, University of Göteborg, Sweden.
J Neurosci Methods. 1995 Dec;63(1-2):13-22. doi: 10.1016/0165-0270(95)00080-1.
A new approach was developed to minimize inevitable damage to nervous and meningeal tissue due to implantation of a sampling tube allowing multiple withdrawal of cerebrospinal fluid (CSF) from the cisterna magna in adult rats. A tube was secured on the atlanto-occipital membrane. Thereafter, a hole was cut through the membrane, allowing flow of CSF from the cisterna magna to the tube. CSF could be sampled repeatedly for at least 1 week. There was no blood-brain barrier damage. The pressure in the cisterna magna remained normal as did the estimated rate of CSF formation. Very few blood cells contaminated the CSF. There was very little evidence of inflammation. The nervous tissue was undamaged as shown by exclusion of a dye-protein complex. The CSF concentrations of the cytosolic neuronal protein neuron-specific enolase (NSE), and of the astrocyte protein S-100 were very low. The pattern of amino acids remained within normal limits. Scanning electron microscopy revealed that clot and reactive changes were restricted to the vicinity of the connecting hole. We conclude that our approach to positioning a tube on the atlanto-occipital membrane and then connecting it to the cisterna magna reproducibly and reliably enables 'atraumatic' multiple sampling of CSF.
开发了一种新方法，以尽量减少成年大鼠因植入采样管从枕大池多次抽取脑脊液（CSF）而对神经和脑膜组织造成的不可避免的损伤。将一根管子固定在寰枕膜上。此后，在膜上切一个孔，使脑脊液从枕大池流入管子。脑脊液可以反复采样至少1周。没有血脑屏障损伤。枕大池内的压力保持正常，脑脊液形成的估计速率也正常。极少有血细胞污染脑脊液。几乎没有炎症迹象。如通过排除染料 - 蛋白质复合物所示，神经组织未受损。细胞溶质神经元蛋白神经元特异性烯醇化酶（NSE）和星形胶质细胞蛋白S - 100的脑脊液浓度非常低。氨基酸模式保持在正常范围内。扫描电子显微镜显示，血凝块和反应性变化仅限于连接孔附近。我们得出结论，我们将管子放置在寰枕膜上然后连接到枕大池的方法可重复且可靠地实现脑脊液的“无创”多次采样。
