Döppenschmitt S A, Scheidel B, Harrison F, Surmann J P
ACC, Analytical Clinical Concepts GmbH, Pfungstadt, Germany.
J Chromatogr B Biomed Appl. 1995 Dec 15;674(2):237-46. doi: 10.1016/0378-4347(95)00317-7.
A method for the simultaneous determination of prednisolone, prednisolone acetate and hydrocortisone has been established to monitor the serum levels of these three compounds in healthy volunteers following intramuscular administration of prednisolone acetate. Serum samples of 0.75 ml were extracted with ethyl acetate after addition of the internal standard, dexamethasone. The compounds were separated using a LiChrosorb Si 60 column and detected by UV absorbance. Specificity, linearity, as well as the repeatability, intermediate-precision and accuracy of the method were established. The lower limit of quantification was 20 ng/ml for prednisolone (C.V. = 14.7%, n = 6) and 5.0 ng/ml for prednisolone acetate (C.V. = 13.9%, n = 6) and hydrocortisone (C.V. = 11.7%, n = 6). Data on the recovery of the compounds and the internal standard are provided. The results of quality control samples determined during routine analysis (n = 114) are presented. Serum levels of the compounds after intramuscular administration of 25 mg of prednisolone acetate are discussed.
已建立一种同时测定泼尼松龙、醋酸泼尼松龙和氢化可的松的方法，用于监测健康志愿者肌肉注射醋酸泼尼松龙后这三种化合物的血清水平。加入内标地塞米松后，用乙酸乙酯萃取0.75 ml血清样本。使用LiChrosorb Si 60柱分离化合物，并通过紫外吸光度进行检测。确定了该方法的特异性、线性以及重复性、中间精密度和准确性。泼尼松龙的定量下限为20 ng/ml（变异系数=14.7%，n=6），醋酸泼尼松龙和氢化可的松的定量下限为5.0 ng/ml（变异系数分别为13.9%，n=6和11.7%，n=6）。提供了化合物和内标的回收率数据。给出了常规分析期间测定的质量控制样品的结果（n=114）。讨论了肌肉注射25 mg醋酸泼尼松龙后化合物的血清水平。
