Collins S P, Ball G, Vonarx E, Hosking C, Shelton M, Hill D, Howden M E
Department of Surgery, Prince Henry Hospital, Little Bay, NSW, Australia.
Clin Exp Allergy. 1996 Jan;26(1):36-42. doi: 10.1111/j.1365-2222.1996.tb00054.x.
The house dust mite has been shown to be an important source of domestic allergens associated with immediate hypersensitivities. The Group I mite allergens Der p I from Dermatophagoides pteronyssinus and Der f I from D. farinae display extensive amino acid sequence homology and have similarities with cysteine protease enzymes.
The availability of the complete amino acid sequences for these allergens allowed us to search for the allergic determinants within these molecules. The aim of the present investigation was to identify any continuous IgE-binding epitopes within these amino acid sequences. We also sought to test the validity of previously reported Der p I peptide epitope sequences.
In order to identify any continuous IgE epitopes, the amino acid sequences of Der p I and Der f I were synthesized as decapeptides overlapping in sequence and coupled to plastic pins. The specific IgE-binding capacity of these peptides was assayed using an enzyme-linked biotin-streptavidin procedure and sera from patients known to be sensitive to these allergens. Previously reported Der p I peptide epitopes were synthesized as free peptides and tested for their ability to inhibit specific IgE binding to allergen extract discs.
None of the pin-coupled Der p I or Der f I peptides was found by the continuous epitope mapping procedure to bind significantly to specific IgE in the sera of hypersensitive patients. The previously reported Der p I peptide epitopes did not inhibit specific IgE binding to mite extract discs.
The specific IgE binding epitopes of the house dust mite allergens Der p I and Der f I are discontinuous in nature.
屋尘螨已被证明是与速发型超敏反应相关的重要室内过敏原来源。来自粉尘螨的I组螨过敏原Der p I和来自屋尘螨的Der f I显示出广泛的氨基酸序列同源性，并且与半胱氨酸蛋白酶具有相似性。
这些过敏原完整氨基酸序列的可得性使我们能够在这些分子中寻找过敏决定簇。本研究的目的是在这些氨基酸序列中鉴定任何连续的IgE结合表位。我们还试图检验先前报道的Der p I肽表位序列的有效性。
为了鉴定任何连续的IgE表位，将Der p I和Der f I的氨基酸序列合成为序列重叠的十肽，并偶联到塑料针上。使用酶联生物素-链霉亲和素方法和已知对这些过敏原敏感的患者血清检测这些肽的特异性IgE结合能力。先前报道的Der p I肽表位合成为游离肽，并测试其抑制特异性IgE与过敏原提取物圆盘结合的能力。
通过连续表位作图程序，未发现针偶联的Der p I或Der f I肽与过敏患者血清中的特异性IgE有明显结合。先前报道的Der p I肽表位不能抑制特异性IgE与螨提取物圆盘的结合。
屋尘螨过敏原Der p I和Der f I的特异性IgE结合表位本质上是不连续的。
