Kaukonen J, Lahtinen I, Laine S, Alitalo K, Palotie A
Department of Clinical Chemistry, University of Helsinki/Laboratory Department of Helsinki University Central Hospital, Finland.
Br J Haematol. 1996 Sep;94(3):455-60.
The growth and maturation of haemopoietic cells is regulated by signal transduction through tyrosine protein kinases. Recently, a novel cytoplasmic tyrosine kinase gene in chromosome X, called Bmx, was identified in human bone marrow RNA. Bmx belongs to a subfamily of tyrosine kinases which are expressed in various haemopoietic cell lineages. We studied Bmx expression using RT-PCR of RNA from fractionated peripheral blood leucocytes, progenitor-enriched fractions of cord blood and from bone marrow or peripheral blood samples from leukaemia patients. Bmx was strongly expressed in haemopoietic tissues and enhanced in neutrophilic granulocytes. Bmx mRNA was also found in CD34-positive progenitor cells from cord blood. All samples (10/10) of patients with acute myeloid leukaemia and (4/4) with chronic myeloid leukaemia showed expression of Bmx. In contrast, none of the samples of acute lymphoid leukaemia (0/8) and only one out of six samples of chronic lymphoid leukaemia expressed Bmx. In conclusion, Bmx expression seems to be associated with myelopoiesis.
造血细胞的生长和成熟是由酪氨酸蛋白激酶介导的信号转导调节的。最近，在人类骨髓RNA中鉴定出一种位于X染色体上的新型细胞质酪氨酸激酶基因，称为Bmx。Bmx属于在各种造血细胞谱系中表达的酪氨酸激酶亚家族。我们使用来自分离的外周血白细胞、富含祖细胞的脐血部分以及白血病患者的骨髓或外周血样本的RNA进行RT-PCR研究Bmx的表达。Bmx在造血组织中强烈表达，并在嗜中性粒细胞中增强。在脐血的CD34阳性祖细胞中也发现了Bmx mRNA。急性髓性白血病患者的所有样本（10/10）和慢性髓性白血病患者的所有样本（4/4）均显示Bmx表达。相比之下，急性淋巴细胞白血病的样本无一（0/8）表达Bmx，慢性淋巴细胞白血病的六个样本中只有一个表达Bmx。总之，Bmx表达似乎与髓系造血有关。
