用铟 - 111标记白细胞对缺血犬心肌炎症反应进行成像。

Imaging of the inflammatory response in ischemic canine myocardium with 111indium-labeled leukocytes.

作者信息

Weiss E S, Ahmed S A, Thakur M L, Welch M J, Coleman R E, Sobel B E

出版信息

Am J Cardiol. 1977 Aug;40(2):195-9. doi: 10.1016/0002-9149(77)90008-x.

Abstract

Myocardial leukocyte infiltration is one hallmark of acute myocardial infarction. In order to detect noninvasively this inflammatory response associated with acute myocardial infarction, we produced coronary occlusion in eight dogs, intravenously administered autologously labeled indium-111 (111In) leukocytes and scintigraphically monitored accumulation of radionuclide in myocardium. Seventy-two hours after coronary occlusion, 111In-labeled white cells accumulated in regions corresponding to myocardial infarcts, and positive images with 111In-labeled leukocytes correlated well with images obtained with technetium-99m pyrophosphate and computer-reconstructed tomograms obtained with nitrogen-13-labeled ammonia. In contrast, two control dogs subjected to sham operation did not exhibit positive 111In-leukocyte images. Scintigraphic results with 111In-labeled leukocytes were verified in vitro by analysis of radioactivity in normal myocardium and in infarcts. Thus, leukocytic infiltration associated with acute myocardial infarction can be detected noninvasively in vivo.

摘要

心肌白细胞浸润是急性心肌梗死的一个标志。为了无创检测与急性心肌梗死相关的这种炎症反应，我们对8只犬进行冠状动脉闭塞，静脉注射自体标记铟-111（111In）白细胞，并通过闪烁显像监测心肌中放射性核素的蓄积。冠状动脉闭塞72小时后，111In标记的白细胞在与心肌梗死相对应的区域蓄积，111In标记白细胞的阳性图像与用锝-99m焦磷酸盐获得的图像以及用氮-13标记氨获得的计算机重建断层图像相关性良好。相比之下，接受假手术的2只对照犬未显示111In白细胞阳性图像。通过分析正常心肌和梗死灶中的放射性，在体外验证了111In标记白细胞的闪烁显像结果。因此，与急性心肌梗死相关的白细胞浸润可在体内无创检测。