Troy G C, Becker M J, Greene R T
Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg 24061-0442, USA.
J Am Vet Med Assoc. 1996 Sep 1;209(5):914-7.
To determine the correlation of seroimmunologic test results between reference and nonreference laboratories.
Retrospective data analysis.
Serum samples obtained from naturally infected dogs and cats were distributed to reference and nonreference laboratories for seroimmunologic testing. Correlation of test results was evaluated by use of nonparametric analysis.
Correlation coefficients were high between laboratory groups for samples tested for feline immunodeficiency virus antibodies, FeLV antigen, and toxoplasmosis antibodies in cats. Results for feline immunodeficiency virus antibody tests from reference laboratories were more likely to be positive than results from nonreference laboratories. Test results for feline infectious peritonitis antibodies, antinuclear antibodies, and Borrelia antibodies in cats were not significant. Coefficient correlations were significant for results of heart-worm antigen, Brucella antibodies, Toxoplasma antibodies, antinuclear antibodies, and rheumatoid factor in dogs. Results for Borrelia antibodies were not correlated between laboratory groups.
Results were highly correlated between reference and nonreference laboratories for 8 of 14 seroimmunologic tests. Seroimmunologic tests for use in cats were less correlated as a group than those for use in dogs. Poor correlation of results between laboratories was attributed to variations in control agents, antigens, reagents, technical expertise, and cutoff values and end-point titers used for diagnosis.
确定参考实验室与非参考实验室血清免疫学检测结果之间的相关性。
回顾性数据分析。
将从自然感染的犬猫身上采集的血清样本分发给参考实验室和非参考实验室进行血清免疫学检测。采用非参数分析评估检测结果的相关性。
在检测猫的猫免疫缺陷病毒抗体、猫白血病病毒抗原和弓形虫抗体的样本中，各实验室组之间的相关系数较高。参考实验室的猫免疫缺陷病毒抗体检测结果比非参考实验室的结果更可能呈阳性。猫传染性腹膜炎抗体、抗核抗体和疏螺旋体抗体的检测结果无统计学意义。犬的心丝虫抗原、布鲁氏菌抗体、弓形虫抗体、抗核抗体和类风湿因子检测结果的系数相关性具有统计学意义。各实验室组之间疏螺旋体抗体的检测结果不相关。
在14项血清免疫学检测中，有8项参考实验室与非参考实验室的结果高度相关。用于猫的血清免疫学检测作为一个整体，其相关性低于用于犬的检测。实验室之间结果的低相关性归因于对照剂、抗原、试剂、技术专长以及用于诊断的临界值和终点滴度的差异。
