Johansson N G, Eriksson S
Medivir AB, Huddinge, Sweden.
Acta Biochim Pol. 1996;43(1):143-60.
The mammalian deoxyribonucleoside kinases thymidine kinase 1 and 2, deoxycytidine kinase and deoxyguanosine kinase phosphorylate deoxyribonucleosides and provide an alternative to de novo synthesis of DNA precursors. Their activities are essential for activation of several chemotherapeutically important nucleoside analogs. These four salvage kinase enzymes exhibit distinct substrate specificities for nucleoside analogs modified in the base and glycon moieties. In this review their. structure-activity relationships are discussed. Alternative routes for phosphorylation of nucleoside analogs are also reviewed, such as the phosphotransfer capacity of 5'-nucleotidase and protein kinases.
哺乳动物的脱氧核糖核苷激酶，即胸苷激酶1和2、脱氧胞苷激酶及脱氧鸟苷激酶，可使脱氧核糖核苷磷酸化，为DNA前体的从头合成提供了一种替代途径。它们的活性对于激活几种具有重要化疗意义的核苷类似物至关重要。这四种补救激酶对在碱基和糖部分修饰的核苷类似物表现出不同的底物特异性。在本综述中，将讨论它们的构效关系。还将综述核苷类似物磷酸化的替代途径，例如5'-核苷酸酶和蛋白激酶的磷酸转移能力。
