Souchier C, Guigard M P, Fonlupt P, Trouillas J
Analytical Cytology Laboratory, Claude Bernard University, Lyon, France.
Anal Quant Cytol Histol. 1996 Jun;18(3):251-60.
An image analysis program was developed to analyze cell density in a whole organ. The method was applied to the evaluation of adrenocorticotrophic hormone (ACTH-storing cells in normal human anterior pituitary in order to help the pathologist to evaluate hyperplasia on small surgical biopsies.
ACTH cells were labeled using the immunoperoxidase technique. Data were systematically acquired on a composite image that made possible two-dimensional cell pattern representation in sequential sections of the organ and measurements of cell density in selected histologic parts.
ACTH-storing cells were located more in the anterior than posterior part and more in the lower than in the upper pituitary part. The probability of observing high-density value even in normal cases was precisely evaluated zone by zone. Moreover, a model was proposed to evaluate mean cell density from small surgical pieces.
The same methodology could be applied to other endocrine cell types in the thyroid, pancreas or intestinal tract when hyperplasia is considered the cause of certain clinical syndromes.
开发了一种图像分析程序，用于分析整个器官中的细胞密度。该方法应用于评估正常人垂体前叶中促肾上腺皮质激素（ACTH）储存细胞，以帮助病理学家评估小手术活检中的增生情况。
使用免疫过氧化物酶技术标记促肾上腺皮质激素（ACTH）细胞。在合成图像上系统地获取数据，这使得在器官的连续切片中进行二维细胞模式表示以及在选定的组织学部位测量细胞密度成为可能。
储存促肾上腺皮质激素（ACTH）的细胞更多位于前部而非后部，更多位于垂体下部而非上部。精确地逐区域评估了即使在正常情况下观察到高密度值的概率。此外，还提出了一个模型来从小手术切片中评估平均细胞密度。
当增生被认为是某些临床综合征的病因时，相同的方法可应用于甲状腺、胰腺或肠道中的其他内分泌细胞类型。
