Atoji Y, Yamamoto Y, Suzuki Y
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Gifu University, Japan.
Arch Histol Cytol. 1996 May;59(2):197-203. doi: 10.1679/aohc.59.197.
Distribution of neurotensin-containing cells and neurotensin receptor-expressing cells was examined in the chicken thymus using a combination of in situ hybridization histochemistry for neurotensin receptor mRNA and immunohistochemistry for neurotensin. Neurotensin receptor mRNA-expressing cells and neurotensin-immunoreactive cells were localized in the medulla. Neurotensin receptor mRNA-expressing cells showed a round or elongated shape, while neurotensin-immunoreactive cells revealed round, ovoid, spindle or reticular shapes. Neurotensin-immunoreactive cells were much more numerous than those displaying labeling for the neurotensin receptor mRNA in males and females. Furthermore, electron microscopic immunohistochemistry for neurotensin showed that immunoreactive cells contained a large number of round or spherical granules which were 250-350 nm in diameter. This evidence indicates that neurotensin is localized in endocrine cells, and that neurotensin receptor mRNA is expressed in epithelial cells. It further suggests the possibility of microenvironmental interaction of neurotensin between endocrine cells and epithelial cells within the chicken thymus.
运用神经降压素受体mRNA原位杂交组织化学与神经降压素免疫组织化学相结合的方法，对鸡胸腺中含神经降压素的细胞和表达神经降压素受体的细胞进行了分布研究。表达神经降压素受体mRNA的细胞和神经降压素免疫反应性细胞定位于髓质。表达神经降压素受体mRNA的细胞呈圆形或细长形，而神经降压素免疫反应性细胞呈现圆形、卵圆形、梭形或网状。在雄性和雌性个体中，神经降压素免疫反应性细胞比显示神经降压素受体mRNA标记的细胞数量多得多。此外，神经降压素的电子显微镜免疫组织化学显示，免疫反应性细胞含有大量直径为250 - 350 nm的圆形或球形颗粒。这一证据表明神经降压素定位于内分泌细胞中，并且神经降压素受体mRNA在上皮细胞中表达。这进一步提示了鸡胸腺内神经降压素在内分泌细胞和上皮细胞之间存在微环境相互作用的可能性。
