Tully T N, Shane S M, Grimes J E, Poston R P, Kearney M T
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA.
Avian Dis. 1996 Apr-Jun;40(2):266-71.
Diagnostic serologic procedures for psittacine chlamydiosis were evaluated using experimentally inoculated cockatiels (Nymphicus hollandicus). Chlamydia psittaci was recovered from subjects on day 16 postinoculation. Seroconversion was confirmed by the direct complement fixation (DCF) test. The blocking enzyme-linked immunosorbent assay was demonstrated to be the most sensitive of three serodiagnostic procedures evaluated from inoculation until day 24 postinfection, with 92% sensitivity and 73% specificity. The DCF test was progressively more sensitive over the duration of the experiment, attaining 100% sensitivity and specificity on the day 24 postinfection. This study confirms the ability of the DCF procedure to adequately detect C. psittaci antibodies. Limitations relating to reproducibility of serologic results under clinical conditions found in this study indicate that a further refinement of tests is required to consistently detect and quantify antibodies.
利用实验接种的鸡尾鹦鹉（虎皮鹦鹉）对鹦鹉衣原体病的诊断血清学程序进行了评估。接种后第16天从受试动物中分离出鹦鹉热衣原体。通过直接补体结合试验（DCF）确认了血清转化。在从接种到感染后第24天评估的三种血清学诊断程序中，阻断酶联免疫吸附试验被证明是最敏感的，敏感性为92%，特异性为73%。在实验过程中，DCF试验的敏感性逐渐提高，在感染后第24天达到100%的敏感性和特异性。本研究证实了DCF程序充分检测鹦鹉热衣原体抗体的能力。本研究发现的临床条件下血清学结果再现性方面的局限性表明，需要进一步改进检测方法以持续检测和定量抗体。
