通过溶解包涵体并从十二烷基肌氨酸钠中重折叠来纯化过量表达的大肠杆菌RNA聚合酶σ因子。 - Suppr

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超能文献

Purification of overproduced Escherichia coli RNA polymerase sigma factors by solubilizing inclusion bodies and refolding from Sarkosyl.

作者信息

Burgess R R

机构信息

McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, 53706, USA.

出版信息

Methods Enzymol. 1996;273:145-9. doi: 10.1016/s0076-6879(96)73014-8.

DOI:10.1016/s0076-6879(96)73014-8

PMID:8791607

Abstract

摘要

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