Fernandes M J, Naffah-Mazzacoratti M G, Cavalheiro E A
Disciplina de Neurologia Experimental, Universidade Federal de São Paulo, Escola Paulista de Medicina, Brazil.
Neurochem Int. 1996 May-Jun;28(5-6):497-500. doi: 10.1016/0197-0186(95)00153-0.
Biochemical abnormalities have been implicated in possible mechanisms underlying the epileptic phenomena. Some of these alterations include changes in the activity of several enzymes present in epileptic tissues. Systemic administration of pilocarpine in rats induces electrographic and behavioral limbic seizures and status epilepticus, that is followed by a transient seizure-free period (silent period). Finally a chronic phase ensues, characterized by spontaneous and recurrent seizures (chronic period), that last for the rest of the animal's life. The present work aimed to study the activity of the enzyme Na+K+ ATPase, in rat hippocampus, during the three phases of this epilepsy model. The enzyme activity was determined at different time points from pilocarpine administration (1 and 24 h of status epilepticus, during the silent and chronic period) using a spectrophotometric assay previously described by Mishra and Delivoria-Papadopoulos [Neurochem. Res. (1988) 13, 765-770]. The results showed decreased enzyme activities during the acute and silent periods and increased Na+K+ ATPase activity during the chronic phase. These data show that changes in Na+K+ ATPase activity could be involved in the appearance of spontaneous and recurrent seizures following brain damage induced by pilocarpine injection.
生化异常被认为是癫痫现象潜在机制的一部分。其中一些改变包括癫痫组织中几种酶活性的变化。给大鼠全身注射毛果芸香碱会诱发脑电图和行为学上的边缘叶癫痫发作及癫痫持续状态，随后会有一个短暂的无癫痫发作期（静止期）。最后进入慢性期，其特征是出现自发性和复发性癫痫发作（慢性期），并持续至动物生命结束。本研究旨在探讨在该癫痫模型的三个阶段中，大鼠海马体中钠钾ATP酶的活性。使用Mishra和Delivoria - Papadopoulos先前描述的分光光度法[《神经化学研究》（1988年）13卷，765 - 770页]，在毛果芸香碱给药后的不同时间点（癫痫持续状态的1小时和24小时、静止期和慢性期）测定酶活性。结果显示，急性期和静止期酶活性降低，而慢性期钠钾ATP酶活性增加。这些数据表明，钠钾ATP酶活性的变化可能与毛果芸香碱注射诱导脑损伤后自发性和复发性癫痫发作的出现有关。
