Naffah-Mazzacoratti M G, Bellíssimo M I, Cavalheiro E A
Disciplina de Neurologia Experimental, Escola Paulista de Medicina, (UNIFESP), São Paulo, Brazil.
Neurochem Int. 1995 Dec;27(6):461-6. doi: 10.1016/0197-0186(95)00053-b.
Pilocarpine (PILO) administered to rats acutely induces status epilepticus (acute period), which is followed by a transient seizure-free period (silent period), and finally by a chronic phase of spontaneous recurrent seizures (chronic period, SRS) that lasts for the rest of animal's life. Hippocampal neurochemical changes following PILO administration include alteration in monoamines and amino acids content during all phases of this epilepsy model. The present work was delineated to study the content of prostaglandins (PG) levels in hippocampus during the three phases of this model. The levels of PG E2, PG F2 alpha and PG D2 were measured by radioimmunoassay 1 h after PILO, 5 h after PILO, during the silent period, and interictally into the chronic period. The results show, in hippocampus of rats, increase of PG F2 alpha and PG D2 during status epilepticus, increase of PG D2 during the silent period and increase of PG E2 and PG D2 during the chronic phase, when compared with control group. These changes match previously reported alteration in monoamines and amino acid levels, showing that altered neurotransmission is accompanied by changes in second messengers and enzyme activity related to PG production during all phases of the epilepsy model.
急性给予大鼠毛果芸香碱（PILO）可诱发癫痫持续状态（急性期），随后是短暂的无癫痫发作期（静止期），最后是自发性反复癫痫发作的慢性期（慢性期，SRS），该慢性期会持续动物的余生。给予PILO后海马神经化学变化包括在该癫痫模型的所有阶段单胺和氨基酸含量的改变。本研究旨在探讨该模型三个阶段海马中前列腺素（PG）水平的含量。在给予PILO后1小时、给予PILO后5小时、静止期以及慢性期的发作间期，通过放射免疫分析法测量PG E2、PG F2α和PG D2的水平。结果显示，与对照组相比，大鼠海马中在癫痫持续状态期间PG F2α和PG D2增加，在静止期PG D2增加，在慢性期PG E2和PG D2增加。这些变化与先前报道的单胺和氨基酸水平的改变相符，表明在癫痫模型的所有阶段，神经传递的改变伴随着与PG产生相关的第二信使和酶活性的变化。
