Benfield M R, Vail A, Waldo F B, Bucy R P, Kohaut E C
Department of Pediatrics, University of Alabama at Birmingham 35233, USA.
Pediatr Nephrol. 1996 Jun;10(3):280-2. doi: 10.1007/BF00866759.
Recombinant human growth hormone (rhGH) improves growth in children after renal transplantation, but may be associated with augmented immune responses. We previously demonstrated that rhGH augments proliferative and cytotoxic responses and interferon-gamma (IFN-gamma) mRNA expression during a mixed leukocyte culture (MLC). In this study, we evaluated 12 pediatric patients after receiving a renal allograft from one of their parents. Peripheral blood mononuclear cells (PBMC) were isolated from patients and cultured with either donor or unrelated third-party PBMC in an MLC. Patients developed significant donor-specific hyporesponsiveness (DSH), however, no correlation was seen between the amount of DSH and graft function. Of the 12 patients, 2 developed augmented responses in the presence of rhGH. rhGH augments proliferation, cytotoxicity, and IFN-gamma expression during an MLC. Some patients develop increased responses to donor-specific alloantigens after renal transplantation. Further study is needed to better determine the significance of this finding.
重组人生长激素（rhGH）可促进肾移植术后儿童的生长，但可能与免疫反应增强有关。我们之前证明，在混合淋巴细胞培养（MLC）过程中，rhGH可增强增殖反应、细胞毒性反应以及干扰素-γ（IFN-γ）mRNA表达。在本研究中，我们评估了12例接受其父母一方肾脏同种异体移植的儿科患者。从患者体内分离出外周血单个核细胞（PBMC），并在MLC中与供体或无关第三方的PBMC一起培养。患者出现了显著的供体特异性低反应性（DSH），然而，DSH的程度与移植肾功能之间未发现相关性。12例患者中，有2例在rhGH存在的情况下出现了增强反应。在MLC过程中，rhGH可增强增殖、细胞毒性以及IFN-γ表达。一些患者在肾移植后对供体特异性同种异体抗原的反应增强。需要进一步研究以更好地确定这一发现的意义。
