Prakash B, Kuosku V, Olsaker I, Gustavsson I, Chowdhary B P
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Chromosome Res. 1996 Apr;4(3):214-7. doi: 10.1007/BF02254962.
Three X chromosome-specific bovine cosmids were used for fluorescence in situ hybridization mapping on reindeer (Rangifer tarandus) chromosomes, to test whether such large genomic clones could be used for comparative mapping across distantly related species. All three cosmids showed distinct unique hybridization sites on the reindeer X. Comparative map locations of these cosmids, together with the relative C-banding and genome size data on the X chromosomes of the two species, provide preliminary indications that the short and long arms of bovine X correspond, respectively, to the long and short arms of the reindeer X. The study also demonstrates that cosmid clones can be used successfully for comparative mapping across species that diverged 35 million years ago.
使用三个X染色体特异性牛黏粒对驯鹿（Rangifer tarandus）染色体进行荧光原位杂交作图，以测试此类大型基因组克隆是否可用于远缘物种间的比较作图。所有三个黏粒在驯鹿X染色体上均显示出独特的杂交位点。这些黏粒的比较图谱位置，以及两个物种X染色体上的相对C带和基因组大小数据，初步表明牛X染色体的短臂和长臂分别对应于驯鹿X染色体的长臂和短臂。该研究还表明，黏粒克隆可成功用于3500万年前分化的物种间的比较作图。
