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1型牛乳头瘤病毒E2刺激DNA复制并不需要转录激活功能。

Transcriptional activation function is not required for stimulation of DNA replication by bovine papillomavirus type 1 E2.

作者信息

Grossel M J, Sverdrup F, Breiding D E, Androphy E J

机构信息

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

出版信息

J Virol. 1996 Oct;70(10):7264-9. doi: 10.1128/JVI.70.10.7264-7269.1996.

DOI:10.1128/JVI.70.10.7264-7269.1996
PMID:8794380
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC190786/
Abstract

Bovine papillomavirus type 1 replication was previously shown to require both the E1 initiator protein and the E2 transactivator protein. We show here that E1, in the absence of E2, is sufficient for low-level bovine papillomavirus type 1 DNA replication in C-33A cells. In addition, studies of genetically isolated E2 point mutants demonstrate that enhancement of replication by E2 does not require its transcriptional activation function. The uncoupling of the E2 functions suggests that stimulation of transcription and replication by enhancer proteins occurs via divergent mechanisms.

摘要

先前已表明，1型牛乳头瘤病毒复制需要E1起始蛋白和E2反式激活蛋白。我们在此表明，在没有E2的情况下，E1足以在C - 33A细胞中进行低水平的1型牛乳头瘤病毒DNA复制。此外，对基因分离的E2点突变体的研究表明，E2对复制的增强作用并不需要其转录激活功能。E2功能的解偶联表明，增强子蛋白对转录和复制的刺激是通过不同机制发生的。

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