用原发性人类免疫缺陷病毒1型分离株感染SK-N-MC细胞（一种CD4阴性神经母细胞瘤细胞系）。
Infection of SK-N-MC cells, a CD4-negative neuroblastoma cell line, with primary human immunodeficiency virus type 1 isolates.
作者信息
Harouse J M, González-Scarano F
机构信息
Graduate Group in Molecular Biology, University of Pennsylvania Medical Center, Philadelphia 19104-6146, USA.
出版信息
J Virol. 1996 Oct;70(10):7290-4. doi: 10.1128/JVI.70.10.7290-7294.1996.
Abstract
Most studies looking at CD4-independent infection have used laboratory strains or their respective molecular clones. To determine whether primary human immunodeficiency virus type 1 isolates could infect CD4-negative cells, we obtained a panel of 23 clinical isolates and characterized the early steps of the viral life cycle in SK-N-MC cells, a CD4-negative, galactosylceramide-positive neuroblastoma cell line. Eight of 23 isolates established a nonproductive infection; entry and postentry restrictions were noted in the others. We were unable to correlate the infectivity for SK-N-MC cells with established biological phenotypes, such as syncytium induction, or with genetic classifications, suggesting that pantropism is an independent biological variable.
摘要
大多数关于不依赖CD4感染的研究都使用了实验室菌株或其各自的分子克隆。为了确定原发性人类免疫缺陷病毒1型分离株是否能感染CD4阴性细胞，我们获得了一组23个临床分离株，并对其在SK-N-MC细胞（一种CD4阴性、半乳糖神经酰胺阳性的神经母细胞瘤细胞系）中病毒生命周期的早期步骤进行了表征。23个分离株中有8个建立了非生产性感染；在其他分离株中发现了进入和进入后限制。我们无法将SK-N-MC细胞的感染性与既定的生物学表型（如合胞体诱导）或基因分类相关联，这表明泛嗜性是一个独立的生物学变量。
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