Kwa H B, Michalides R J, Dijkman J H, Mooi W J
Department of Pulmonology C-3-P, University Hospital Leiden, The Netherlands.
Lung Cancer. 1996 Jun;14(2-3):207-17. doi: 10.1016/0169-5002(96)00547-8.
Expression of the neural cell adhesion molecule, NCAM, in frozen sections has been associated with decreased postoperative survival in non-small cell lung carcinoma. Of the various isoforms of NCAM described, the highly sialylated isoform plays a role in the migration of embryonal cells from the neural crest and is expressed by highly malignant tumours such as small cell lung carcinomas. We investigated the clinical significance of expression of this NCAM isoform as a prognostic factor in a series of 96 non-small cell lung carcinomas resected with curative intent. We also evaluated the effect of microwave pre-treatment of formalin-fixed, paraffin-embedded sections on the NCAM immunostaining and related the outcome to the postoperative clinical course of disease. In addition, in an attempt to extend our search for possible molecular markers of unfavourable prognosis in lung cancer, we evaluated increased immunostaining for p53 and cyclin D1 in the same series. We did not find a significant relation between expression of NCAM or its highly sialylated isoform and the length of postoperative survival. The numbers of positive cases (9 and 14, respectively) were relatively low. Increased p53 and cyclin D1 immunostaining (50 and 55 of the 96 tumours) failed to show a significant relation with postoperative survival. In our material, tumour stage was the only significant prognostic factor.
神经细胞黏附分子（NCAM）在冰冻切片中的表达与非小细胞肺癌患者术后生存率降低有关。在已描述的NCAM的各种亚型中，高度唾液酸化的亚型在神经嵴胚胎细胞迁移中起作用，并在诸如小细胞肺癌等高度恶性肿瘤中表达。我们研究了这种NCAM亚型的表达作为预后因素在一系列96例根治性切除的非小细胞肺癌中的临床意义。我们还评估了对福尔马林固定、石蜡包埋切片进行微波预处理对NCAM免疫染色的影响，并将结果与疾病的术后临床病程相关联。此外，为了扩大我们对肺癌不良预后可能分子标志物的研究，我们在同一组病例中评估了p53和细胞周期蛋白D1免疫染色增强的情况。我们未发现NCAM或其高度唾液酸化亚型的表达与术后生存时间之间存在显著关联。阳性病例数（分别为9例和14例）相对较少。p53和细胞周期蛋白D1免疫染色增强（96例肿瘤中有50例和55例）与术后生存情况未显示出显著关联。在我们的研究材料中，肿瘤分期是唯一显著的预后因素。
