新西兰麻黄化叶植原体两个16S rRNA基因中的序列异质性。
Sequence heterogeneity in the two 16S rRNA genes of Phormium yellow leaf phytoplasma.
作者信息
Liefting L W, Andersen M T, Beever R E, Gardner R C, Forster R L
机构信息
Horticulture and Food Research Institute of New Zealand Ltd., Auckland, New Zealand.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3133-9. doi: 10.1128/aem.62.9.3133-3139.1996.
Abstract
Phormium yellow leaf (PYL) phytoplasma causes a lethal disease of the monocotyledon, New Zealand flax (Phormium tenax). The 16S rRNA genes of PYL phytoplasma were amplified from infected flax by PCR and cloned, and the nucleotide sequences were determined. DNA sequencing and Southern hybridization analysis of genomic DNA indicated the presence of two copies of the 16S rRNA gene. The two 16S rRNA genes exhibited sequence heterogeneity in 4 nucleotide positions and could be distinguished by the restriction enzymes BpmI and BsrI. This is the first record in which sequence heterogeneity in the 16S rRNA genes of a phytoplasma has been determined by sequence analysis. A phylogenetic tree based on 16S rRNA gene sequences showed that PYL phytoplasma is most closely related to the stolbur and German grapevine yellows phytoplasmas, which form the stolbur subgroup of the aster yellows group. This phylogenetic position of PYL phytoplasma was supported by 16S/23S spacer region sequence data.
摘要
新西兰麻黄化叶植原体可引发单子叶植物新西兰麻（Phormium tenax）的一种致死性病害。通过PCR从受感染的亚麻中扩增并克隆了新西兰麻黄化叶植原体的16S rRNA基因，并测定了其核苷酸序列。基因组DNA的DNA测序和Southern杂交分析表明存在两个16S rRNA基因拷贝。这两个16S rRNA基因在4个核苷酸位置上表现出序列异质性，并且可以通过限制性内切酶BpmI和BsrI进行区分。这是首次通过序列分析确定植原体16S rRNA基因序列异质性的记录。基于16S rRNA基因序列构建的系统发育树表明，新西兰麻黄化叶植原体与形成翠菊黄化组翠菊黄化亚组的 stolbur 和德国葡萄黄化植原体关系最为密切。16S/23S间隔区序列数据支持了新西兰麻黄化叶植原体的这一系统发育位置。
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本文引用的文献
1
Presence of two sets of ribosomal genes in phytopathogenic mollicutes.植物病原柔膜菌中存在两套核糖体基因。
Appl Environ Microbiol. 1994 Sep;60(9):3409-12. doi: 10.1128/aem.60.9.3409-3412.1994.
2
Genetic Interrelatedness among Clover Proliferation Mycoplasmalike Organisms (MLOs) and Other MLOs Investigated by Nucleic Acid Hybridization and Restriction Fragment Length Polymorphism Analyses.核酸杂交和限制性片段长度多态性分析研究三叶草增殖类菌原体（MLO）及其它 MLO 间的遗传相关性。
Appl Environ Microbiol. 1991 Dec;57(12):3565-9. doi: 10.1128/aem.57.12.3565-3569.1991.
3
A DNA extraction method that allows reliable PCR amplification of MLO DNA from "difficult" plant host species.
PCR Methods Appl. 1994 Aug;4(1):56-8. doi: 10.1101/gr.4.1.56.
4
Lessons from an evolving rRNA: 16S and 23S rRNA structures from a comparative perspective.从不断演变的核糖体RNA中汲取的经验教训：从比较的角度看16S和23S核糖体RNA结构
Microbiol Rev. 1994 Mar;58(1):10-26. doi: 10.1128/mr.58.1.10-26.1994.
5
Characterization of the 16S rRNA genes from Mycoplasma sp. strain F38 and development of an identification system based on PCR.来自支原体属菌株F38的16S rRNA基因的特征分析及基于PCR的鉴定系统的开发。
J Bacteriol. 1994 May;176(9):2577-86. doi: 10.1128/jb.176.9.2577-2586.1994.
6
Phylogeny of mycoplasmalike organisms (phytoplasmas): a basis for their classification.类菌原体（植原体）的系统发育：其分类的基础。
J Bacteriol. 1994 Sep;176(17):5244-54. doi: 10.1128/jb.176.17.5244-5254.1994.
7
The Ribosomal Database Project.核糖体数据库项目
Nucleic Acids Res. 1994 Sep;22(17):3485-7. doi: 10.1093/nar/22.17.3485.
8
Phylogenetic classification of phytopathogenic mollicutes by sequence analysis of 16S ribosomal DNA.基于16S核糖体DNA序列分析的植物病原柔膜菌纲的系统发育分类
Int J Syst Bacteriol. 1994 Jul;44(3):440-6. doi: 10.1099/00207713-44-3-440.
9
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
10
Mycoplasmas (Mollicutes) have a low number of rRNA genes.支原体（柔膜菌纲）的核糖体RNA基因数量较少。
J Bacteriol. 1984 Apr;158(1):376-8. doi: 10.1128/jb.158.1.376-378.1984.
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