来自乳酸乳球菌乳脂亚种W56的噬菌体抗性系统LlaBI限制修饰系统的克隆与分析。
Cloning and analysis of the restriction-modification system LlaBI, a bacteriophage resistance system from Lactococcus lactis subsp. cremoris W56.
作者信息
Nyengaard N R, Falkenberg-Klok J, Josephsen J
机构信息
Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3494-8. doi: 10.1128/aem.62.9.3494-3498.1996.
Abstract
The genes coding for the type II restriction-modification (R/M) system LlaBI, which recognized the sequence 5'-C decreases TRYAG-3', have been cloned from a plasmid in Lactococcus lactis subsp. cremoris W56 and sequenced. The DNA sequence predicts an endonuclease of 299 amino acids (33 kDa) and a methylase of 580 amino acids (65 kDa). A 4.0-kb HindIII fragment in pSA3 was able to restrict bacteriophages, showing that the cloned R/M system can function as a phage defense mechanism in L. lactis.
摘要
编码II型限制-修饰（R/M）系统LlaBI的基因已从乳酸乳球菌亚种cremoris W56的一个质粒中克隆并测序，该系统识别序列5'-C decreases TRYAG-3'。DNA序列预测有一个299个氨基酸（33 kDa）的内切核酸酶和一个580个氨基酸（65 kDa）的甲基化酶。pSA3中的一个4.0 kb HindIII片段能够限制噬菌体，表明克隆的R/M系统可在乳酸乳球菌中作为一种噬菌体防御机制发挥作用。
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