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Appl Environ Microbiol. 1996 Sep;62(9):3494-8. doi: 10.1128/aem.62.9.3494-3498.1996.
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本文引用的文献

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The Lactococcal Plasmid pNP40 Encodes a Third Bacteriophage Resistance Mechanism, One Which Affects Phage DNA Penetration.乳球菌质粒细胞 pNP40 编码第三种噬菌体抗性机制，该机制影响噬菌体 DNA 穿透。
Appl Environ Microbiol. 1996 Feb;62(2):676-9. doi: 10.1128/aem.62.2.676-679.1996.
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Appl Microbiol. 1975 Jun;29(6):807-13. doi: 10.1128/am.29.6.807-813.1975.
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Divergence of Genomic Sequences between Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris.乳球菌乳亚种和乳球菌乳亚种之间基因组序列的差异。
Appl Environ Microbiol. 1992 Dec;58(12):4045-7. doi: 10.1128/aem.58.12.4045-4047.1992.
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High-Frequency Transformation, by Electroporation, of Lactococcus lactis subsp. cremoris Grown with Glycine in Osmotically Stabilized Media.在渗透压稳定的培养基中用甘氨酸培养的乳球菌乳亚种经电穿孔高频转化。
Appl Environ Microbiol. 1989 Dec;55(12):3119-23. doi: 10.1128/aem.55.12.3119-3123.1989.
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Cloning and DNA sequence analysis of two abortive infection phage resistance determinants from the lactococcal plasmid pNP40.来自乳球菌质粒pNP40的两个流产感染噬菌体抗性决定簇的克隆及DNA序列分析。
Appl Environ Microbiol. 1995 Dec;61(12):4321-8. doi: 10.1128/aem.61.12.4321-4328.1995.
6
ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503: cloning and characterization of two ScrFI methylase genes.乳酸乳球菌乳脂亚种UC503的ScrFI限制修饰系统：两个ScrFI甲基化酶基因的克隆与鉴定
Appl Environ Microbiol. 1993 Mar;59(3):777-85. doi: 10.1128/aem.59.3.777-785.1993.
7
LlaAI and LlaBI, two type-II restriction endonucleases from Lactococcus lactis subsp. cremoris W9 and W56 recognizing, respectively, 5'-/GATC-3' and 5'-C/TRYAG-3'.LlaAI和LlaBI是乳酸乳球菌亚种cremoris W9和W56中的两种II型限制性内切酶，它们分别识别5'-/GATC-3'和5'-C/TRYAG-3'。
Gene. 1993 Dec 22;136(1-2):371-2. doi: 10.1016/0378-1119(93)90499-s.
8
Cloning and sequencing of LlaDCHI [corrected] restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system.来自乳酸乳球菌的LlaDCHI[已校正]限制/修饰基因的克隆与测序以及该系统与肺炎链球菌DpnII系统的相关性。
Appl Environ Microbiol. 1995 Jun;61(6):2193-202. doi: 10.1128/aem.61.6.2193-2202.1995.
9
Gene pvuIIW: a possible modulator of PvuII endonuclease subunit association.基因pvuIIW：PvuII核酸内切酶亚基关联的一种可能调节因子。
Gene. 1995 May 19;157(1-2):193-9. doi: 10.1016/0378-1119(94)00704-v.
10
Restriction-modification systems in Lactococcus lactis.乳酸乳球菌中的限制修饰系统。
Gene. 1995 May 19;157(1-2):13-8. doi: 10.1016/0378-1119(95)91235-r.

来自乳酸乳球菌乳脂亚种W56的噬菌体抗性系统LlaBI限制修饰系统的克隆与分析。

Cloning and analysis of the restriction-modification system LlaBI, a bacteriophage resistance system from Lactococcus lactis subsp. cremoris W56.

作者信息

Nyengaard N R, Falkenberg-Klok J, Josephsen J

机构信息

Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.

出版信息

Appl Environ Microbiol. 1996 Sep;62(9):3494-8. doi: 10.1128/aem.62.9.3494-3498.1996.

DOI:10.1128/aem.62.9.3494-3498.1996
PMID:8795244
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC168150/
Abstract

The genes coding for the type II restriction-modification (R/M) system LlaBI, which recognized the sequence 5'-C decreases TRYAG-3', have been cloned from a plasmid in Lactococcus lactis subsp. cremoris W56 and sequenced. The DNA sequence predicts an endonuclease of 299 amino acids (33 kDa) and a methylase of 580 amino acids (65 kDa). A 4.0-kb HindIII fragment in pSA3 was able to restrict bacteriophages, showing that the cloned R/M system can function as a phage defense mechanism in L. lactis.

摘要

编码II型限制-修饰（R/M）系统LlaBI的基因已从乳酸乳球菌亚种cremoris W56的一个质粒中克隆并测序，该系统识别序列5'-C decreases TRYAG-3'。DNA序列预测有一个299个氨基酸（33 kDa）的内切核酸酶和一个580个氨基酸（65 kDa）的甲基化酶。pSA3中的一个4.0 kb HindIII片段能够限制噬菌体，表明克隆的R/M系统可在乳酸乳球菌中作为一种噬菌体防御机制发挥作用。