一种用于哈维氏弧菌的选择性和鉴别性培养基。
A selective and differential medium for Vibrio harveyi.
作者信息
Harris L, Owens L, Smith S
机构信息
Department of Biomedical and Tropical Veterinary Sciences, James Cook University of North Queensland, Townsville, Australia.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3548-50. doi: 10.1128/aem.62.9.3548-3550.1996.
A new medium, termed Vibrio harveyi agar, has been developed for the isolation and enumeration of V. harveyi. It is possible to differentiate V. harveyi colonies from the colonies of strains representing 15 other Vibrio species with this medium. This medium has been shown to inhibit the growth of two strains of marine Pseudomonas spp. and two strains of marine Flavobacterium spp. but to allow the growth of Photobacterium strains. Colonies displaying typical V. harveyi morphology were isolated from the larval rearing water of a commercial prawn hatchery with V. harveyi agar as a primary isolation medium and were positively identified, by conventional tests, as V. harveyi. This agar displays great potential as a primary isolation medium and offers significant advantages over thiosulfate-citrate-bile salts-sucrose agar as a medium for differentiating V. harveyi from other marine and estuarine Vibrio species.
一种名为哈维氏弧菌琼脂的新型培养基已被开发出来，用于分离和计数哈维氏弧菌。利用这种培养基能够将哈维氏弧菌菌落与代表其他15种弧菌属菌株的菌落区分开来。已证明这种培养基可抑制两株海洋假单胞菌属菌株和两株海洋黄杆菌属菌株的生长，但能让发光杆菌属菌株生长。以哈维氏弧菌琼脂作为主要分离培养基，从一家商业对虾孵化场的幼体培育水中分离出呈现典型哈维氏弧菌形态的菌落，并通过传统测试将其明确鉴定为哈维氏弧菌。这种琼脂作为主要分离培养基显示出巨大潜力，并且作为一种用于区分哈维氏弧菌与其他海洋和河口弧菌属物种的培养基，它比硫代硫酸盐 - 柠檬酸盐 - 胆盐 - 蔗糖琼脂具有显著优势。
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