培养基成分和代谢抑制剂对黑曲霉菌丝体产葡萄糖氧化酶的影响。

Influence of medium components and metabolic inhibitors on glucose oxidase production by Aspergillus niger mycelium.

作者信息

Gromada A, Fiedurek J

机构信息

Department of Industrial Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.

出版信息

Acta Microbiol Pol. 1996;45(1):37-43.

PMID:8795255

Abstract

The effect of some medium components and metabolic inhibitors on glucose oxidase (GOD) production by mutant Aspergillus niger was studied in shaken culture. Altering the composition of the basal medium, particularly substitution of (NH4)2HPO4 for NaNO3, and lack of Mg2+ ions caused an increase in GOD activity by 269.6%. The highest yield of extracellular GOD activity of A. niger mycelium was obtained in the presence of hematin (1 mM), choline (40 mM) and Tween 80 (0.1%), which improved the activity of this enzyme by about 31.4-53.9%. A significant increase (68.3%) in intracellular GOD activity was found in the presence of sodium orthovanadate (1 mM). The time course of growth and enzyme production by A. niger in the optimized medium is also reported.

摘要

在摇瓶培养中研究了某些培养基成分和代谢抑制剂对突变黑曲霉产生葡萄糖氧化酶（GOD）的影响。改变基础培养基的组成，特别是用(NH4)2HPO4替代NaNO3以及缺乏Mg2+离子，使GOD活性提高了269.6%。在存在血红素（1 mM）、胆碱（40 mM）和吐温80（0.1%）的情况下，黑曲霉菌丝体的胞外GOD活性获得最高产量，该酶的活性提高了约31.4 - 53.9%。在存在原钒酸钠（1 mM）的情况下，细胞内GOD活性显著增加（68.3%）。还报道了黑曲霉在优化培养基中生长和产酶的时间进程。