Malek A, Sager R, Altermatt H J, Gaeng D, Leiser R, Schneider H
Department of Obstetrics and Gynecology, University of Berne, Switzerland.
J Soc Gynecol Investig. 1996 May-Jun;3(3):113-20.
To assess the glycolytic activity of human placental tissue in the third trimester as measured by glucose consumption and lactate production under different conditions of in vitro incubation.
An incubation technique was used to study the metabolic activity of the human placenta by comparing large blocks (0.3 cm3, T1) and small fragments (explants, 0.03 cm3, T2). Placentas were obtained from premature (28-33 weeks) and term (39-41 weeks) deliveries. In addition, different experimental conditions were used to investigate the influence of incubation medium (Earle's buffer and cell culture medium NCTC = 135), medium oxygen pressure (PO2) (400 and 30 mmHg), and regional sampling of placental tissue (central, intermediate, and peripheral). All media contained glucose (1 g/L). The tissue (2-3 g/25 mL medium, pH 7.2-7.4) was incubated for 3 hours at 37C. 3H-inulin was used for the determination of the extracellular space.
Incubation of both tissue forms yielded a higher metabolic activity as measured by glucose consumption and lactate production when incubated with Earle's buffer compared with incubations with NCTC medium. In general, the metabolic activity was consistently higher for small fragments compared with the large blocks. Extracellular space values found for large fragments (25-31%) were significantly lower than for small pieces (39-46%), indicating that an equilibration of the medium with the extracellular space is inadequate with large fragments. Incubation with small fragments showed that 1) there is a tendency for higher metabolic activity during incubation with lower PO2, 2) the metabolically most active part of the placenta is the intermediate tissue region, and 3) placental metabolic activity was significantly higher at 28-33 weeks (n = 5) than at term (n = 6). These differences were not seen with large fragment incubations.
The smaller tissue fragments are preferable for the in vitro incubation study of placental glucose metabolism. Apparently, there are differences in the metabolic activity with regard to the placental tissue region and gestational age.
通过在不同体外培养条件下测量葡萄糖消耗和乳酸生成，评估妊娠晚期人胎盘组织的糖酵解活性。
采用培养技术，通过比较大块组织（0.3 cm³，T1）和小碎片组织（外植体，0.03 cm³，T2）来研究人胎盘的代谢活性。胎盘取自早产（28 - 33周）和足月分娩（39 - 41周）。此外，采用不同的实验条件来研究培养介质（Earle缓冲液和细胞培养基NCTC = 135）、培养基氧分压（PO₂）（400和30 mmHg）以及胎盘组织区域采样（中央、中间和外周）的影响。所有培养基均含有葡萄糖（1 g/L）。将组织（2 - 3 g/25 mL培养基，pH 7.2 - 7.4）在37℃下培养3小时。使用³H - 菊粉测定细胞外间隙。
与使用NCTC培养基培养相比，两种组织形式在与Earle缓冲液一起培养时，通过葡萄糖消耗和乳酸生成测量的代谢活性更高。一般来说，小碎片组织的代谢活性始终高于大块组织。大块组织的细胞外间隙值（25 - 31%）显著低于小碎片组织（39 - 46%），表明大块组织与细胞外间隙的培养基平衡不充分。小碎片组织培养显示：1）在较低PO₂培养期间有代谢活性较高的趋势；2）胎盘代谢最活跃的部分是中间组织区域；3）胎盘代谢活性在28 - 33周（n = 5）时显著高于足月时（n = 6）。大块组织培养未观察到这些差异。
较小的组织碎片更适合用于胎盘葡萄糖代谢的体外培养研究。显然，胎盘组织区域和胎龄在代谢活性方面存在差异。
