Reiber W, Nöschel H, Schröder S, Müller B, Gross W, Michels W
Klinik und Poliklinik für Frauenheilkunde und Geburtshilfe, Bereiches Medizin der Friedrich-Schiller-Universität Jena.
Zentralbl Gynakol. 1990;112(4):207-14.
The aim of the experiments was the in vitro-investigation of metabolic reactions of human placentae under different oxygen supply, particularly when oxygenation followed a period of hypoxia. A perfusion system was used with both circuits of a whole placenta being recirculated over 2 hours with blood-containing perfusate. In group A 17 placentae were perfused under normoxic conditions; in group B 10 perfusions were carried out under hypoxia. In group C 8 placentae were oxygenated after 60 minutes of hypoxic perfusion. (All results are given in mumol/g wet weight/h). In group B glucose uptake (2.95 +/- 1.11) was slightly increased compared to group A (2.59 +/- 1.68), while lactate production was markedly higher (7.69 +/- 1.04 versus 3.56 +/- 1.53 in group A). Oxygen consumption was smaller under hypoxia. Pyruvate showed no significant changes over the 2 hours in all groups. In group C the results of the first hour resembled those of group B. After reoxygenation these placentae showed a smaller oxygen consumption compared to group A, but the highest glucose uptake (4.35 +/- 0.72), whereas lactate production (5.26 +/- 1.86) was lowered compared to group B. The smaller oxygen consumption could be explained by a deterioration of mitochondriae and hence a decreased capacity of oxydative metabolism. The higher glucose uptake could reflect the ability of placental tissue to restore other energy-sources (e.g. proteins) which were affected under the previous hypoxia. Further investigations are required to answer questions of placental function and its disturbances.
这些实验的目的是在体外研究人胎盘在不同氧气供应条件下的代谢反应，特别是在经历一段缺氧期后再进行氧合时的情况。使用了一个灌注系统，整个胎盘的两个循环都用含血灌注液再循环2小时。A组17个胎盘在常氧条件下进行灌注；B组10次灌注在缺氧条件下进行。C组8个胎盘在缺氧灌注60分钟后进行氧合。（所有结果均以微摩尔/克湿重/小时给出）。与A组（2.59±1.68）相比，B组的葡萄糖摄取量（2.95±1.11）略有增加，而乳酸生成量明显更高（分别为7.69±1.04和A组的3.56±1.53）。缺氧时氧气消耗量较小。丙酮酸在所有组中2小时内均无显著变化。C组第一小时的结果与B组相似。再氧合后，这些胎盘的氧气消耗量比A组小，但葡萄糖摄取量最高（4.35±0.72），而乳酸生成量（5.26±1.86）比B组降低。氧气消耗量较小可能是由于线粒体功能恶化，从而导致氧化代谢能力下降。较高的葡萄糖摄取量可能反映了胎盘组织恢复受先前缺氧影响的其他能量来源（如蛋白质）的能力。需要进一步研究以回答胎盘功能及其紊乱的问题。
