Luckhardt M, Leiser R, Kingdom J, Malek A, Sager R, Kaisig C, Schneider H
Department of Obstetrics and Gynaecology, University of Berne, Switzerland.
J Soc Gynecol Investig. 1996 Jul-Aug;3(4):166-71.
To estimate the in vivo dimensions of the fetal villous tree of the normal term placenta.
Dual-circuit perfusion-fixation of a cotyledon from eight normal term placentas was performed with random intra-cotyledon tissue sampling. Stereologic methods were used to derive estimates of villous (intermediate and terminal) surface area and volume, and star volume (a measure of villous volume).
Villous surface area (mean 20.9 m2 [standard deviation 1.8]), capillary surface area (12.8 m2 [1.5]), villous volume (469 mL [40]), and capillary volume (80 mL [10]) values were all approximately 50% higher than reported previously. Star volume estimates ranged from 480 to 1350 microns 3.
Tissue perfusion-fixation more accurately reconstructs the in vivo state, resulting in higher reference values than previously thought for the fetal villous tree dimensions. Up to one-quarter of fetoplacental blood volume is likely to be accommodated within the placenta at term.
评估足月正常胎盘胎儿绒毛树的体内尺寸。
对8个足月正常胎盘的一个小叶进行双循环灌注固定，并在小叶内随机进行组织采样。采用体视学方法估算绒毛（中间绒毛和终末绒毛）表面积、体积以及星状体积（一种绒毛体积的测量指标）。
绒毛表面积（均值20.9平方米[标准差1.8]）、毛细血管表面积（12.8平方米[1.5]）、绒毛体积（469毫升[40]）以及毛细血管体积（80毫升[10]）均比之前报道的值高出约50%。星状体积估算值在480至1350立方微米之间。
组织灌注固定能更准确地重建体内状态，使得胎儿绒毛树尺寸的参考值比之前认为的更高。足月时，胎盘可能容纳高达四分之一的胎儿-胎盘血容量。
