Vandermolen D T, Kauma S W, Turner T T
Department of Obstetrics and Gynecology, Medical College of Virginia, Richmond, USA.
J Soc Gynecol Investig. 1996 Jul-Aug;3(4):172-8.
To test the hypothesis that interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) regulate granulocyte colony-stimulating factor (G-CSF) production by human placental villous core mesenchymal cells.
Villous core mesenchymal cells were isolated from placentas at 14-20 weeks' gestation and cultured in vitro. Cells were treated with IL-1 beta or TNF-alpha in dose-response and time-course studies. We measured G-CSF mRNA expression by Northern blot analysis and G-CSF protein production by enzyme-linked immunosorbent assay of the conditioned media.
Unstimulated mesenchymal cells expressed negligible G-CSF. Steady-state G-CSF mRNA expression was maximal 3-6 hours after IL-1 beta treatment and 6-18 hours after TNF-alpha treatment. Each cytokine induced G-CSF protein production in dose-and time-dependent manners, with IL-1 beta more potent than TNF-alpha. The G-CSF mRNA expression and G-CSF protein production induced by the combination of both cytokines exceeded that induced by either cytokine alone.
Interleukin-1 beta and TNF-alpha stimulate G-CSF production by placental villous core mesenchymal cells in vitro. These results identify a potential mechanism by which villous core mesenchymal cells mediate, in part, the placental response to these two cytokines.
检验白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）调节人胎盘绒毛核心间充质细胞产生粒细胞集落刺激因子（G-CSF）这一假说。
从妊娠14 - 20周的胎盘分离绒毛核心间充质细胞并进行体外培养。在剂量反应和时间进程研究中用IL-1β或TNF-α处理细胞。我们通过Northern印迹分析测量G-CSF mRNA表达，并通过对条件培养基进行酶联免疫吸附测定来测量G-CSF蛋白产生。
未受刺激的间充质细胞表达的G-CSF可忽略不计。IL-1β处理后3 - 6小时和TNF-α处理后6 - 18小时，稳态G-CSF mRNA表达达到最大值。每种细胞因子均以剂量和时间依赖性方式诱导G-CSF蛋白产生，IL-1β比TNF-α更有效。两种细胞因子联合诱导的G-CSF mRNA表达和G-CSF蛋白产生超过单独一种细胞因子诱导的水平。
白细胞介素-1β和肿瘤坏死因子-α在体外刺激胎盘绒毛核心间充质细胞产生G-CSF。这些结果确定了绒毛核心间充质细胞部分介导胎盘对这两种细胞因子反应的潜在机制。
