Goshen R, Schreck H, Komitowski D, Karnaoukhova S, Gonik B, Weinstein D, de Groot N, Hochberg A
Department of Biological Chemistry, Silberman Institute of Life Sciences, Hebrew University, Jerusalem, Israel.
J Soc Gynecol Investig. 1996 Jul-Aug;3(4):209-15.
To define the biology of the tumor-host cell interaction with regard to cellular kinetics and morphologic changes during cell-cell interaction in an in vitro model of trophoblastic neoplasia.
Using a coculture in vitro system of cytotrophoblasts and choriocarcinoma cells, we investigated the cellular kinetics and the morphologic changes in these interacting cells. A fully automatic time-lapse image system was used to record phase contrast images of the cocultured cells in a tissue culture chamber. To examine cytoskeletal structure, immunofluorescent-labeled antibodies against intermediate filaments were used. Slides were examined with a confocal laser scanning microscope and subjected to computed analysis.
The choriocarcinoma cells attract normal cytotrophoblasts using what resembles pseudopodia to engulf the latter cells and thus form slow-growing colonies. In this process, new hybrid cells are formed, which can be differentiated from their original contributors by morphologic characteristics.
This phenomenon supports our previous biochemical and molecular data on the role of cell-cell interaction in the complex process of cytotrophoblast transformation and the development of gestational trophoblastic neoplasia.
在滋养层细胞瘤体外模型中，从细胞动力学和细胞间相互作用期间的形态学变化方面来界定肿瘤-宿主细胞相互作用的生物学特性。
利用细胞滋养层细胞与绒毛膜癌细胞的体外共培养系统，我们研究了这些相互作用细胞的细胞动力学及形态学变化。使用全自动延时图像系统记录组织培养室中共培养细胞的相差图像。为检测细胞骨架结构，使用了针对中间丝的免疫荧光标记抗体。载玻片用共聚焦激光扫描显微镜检查并进行计算机分析。
绒毛膜癌细胞利用类似伪足的结构吸引正常细胞滋养层细胞以吞噬后者，从而形成生长缓慢的集落。在此过程中，形成了新的杂交细胞，可根据形态学特征将其与其原始细胞区分开来。
这一现象支持了我们先前关于细胞间相互作用在细胞滋养层细胞转化及妊娠滋养层细胞瘤发展这一复杂过程中作用的生化和分子数据。
