Ohmiya Y, Hirano T, Ohashi M
PRESTO, JRDC, Institute of Physical and Chemical Research (RIKEN), Wako, Japan.
FEBS Lett. 1996 Apr 8;384(1):83-6. doi: 10.1016/0014-5793(96)00288-8.
Six chimeric mutants between Hotaria parvula (lambda max = 568 nm) and Pyrocoelia miyako (lambda max = 550 nm) luciferases were reconstructed to determine the structural origin of the color differences in firefly luciferase. Based on light-emitting color, five chimeric luciferases could be divided into two groups: the three green-emitting mutants, classified as P. miyako luciferase, and the two yellow-emitting mutants, classified as H. parvula luciferase. Their common fragments between Val-209 and Ala-318 within each group contain the active site for the color differences.
构建了六株细小萤光萤光素酶（λmax = 568 nm）和宫古萤光萤光素酶（λmax = 550 nm）之间的嵌合突变体，以确定萤火虫萤光素酶颜色差异的结构起源。基于发光颜色，五个嵌合萤光素酶可分为两组：三个发出绿色光的突变体，归类为宫古萤光萤光素酶；两个发出黄色光的突变体，归类为细小萤光萤光素酶。每组中位于Val-209和Ala-318之间的共同片段包含颜色差异的活性位点。
