Ohmiya Y, Ohba N, Toh H, Tsuji F
PRESTO, JRDC, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Photochem Photobiol. 1995 Aug;62(2):309-13. doi: 10.1111/j.1751-1097.1995.tb05273.x.
Cloning and sequence analysis of cDNA for the luciferases of Pyrocoelia miyako and Hotaria parvula were carried out (GenBank accession numbers L39928 and L39929, respectively). The amino acid sequence, deduced from the nucleotide sequence, showed P. miyako luciferase to consist of 548 amino acid residues with a molecular weight of 60,955, while the luciferase of H. parvula consisted of 548 amino acid residues with a molecular weight of 60,364. Pyrocoelia miyako luciferase showed 82.1% homology with the luciferase of Photinus pyralis and less than 70% homology with other firefly luciferases, whereas H. parvula luciferase showed 98%, 82.5% and 81.2% homology with the luciferases of Luciola mingrelica, Luciola lateralis and Luciola, cruciata respectively. Two regions in the enzymes were found to be highly conserved. The amino acid sequences were used to construct a phylogenetic tree, which showed that the fireflies could be divided into two groups.
对宫古扁萤（Pyrocoelia miyako）和姬红萤（Hotaria parvula）荧光素酶的cDNA进行了克隆和序列分析（GenBank登录号分别为L39928和L39929）。从核苷酸序列推导的氨基酸序列显示，宫古扁萤荧光素酶由548个氨基酸残基组成，分子量为60,955，而姬红萤荧光素酶由548个氨基酸残基组成，分子量为60,364。宫古扁萤荧光素酶与萤火虫（Photinus pyralis）荧光素酶的同源性为82.1%，与其他萤火虫荧光素酶的同源性低于70%，而姬红萤荧光素酶与明纹扁萤（Luciola mingrelica）、日本扁萤（Luciola lateralis）和红胸扁萤（Luciola cruciata）荧光素酶的同源性分别为98%、82.5%和81.2%。发现这两种酶中有两个区域高度保守。利用氨基酸序列构建了系统发育树，结果表明萤火虫可分为两组。
